食品中4种交链孢毒素对肝脏细胞的联合毒性  

Combined Toxicity with Four Alternaria Toxins in Food of BRL-3A Cells

作　　者：于松 邹连蓬 赵佳伟 朱怡平 薛黎明 卢大胜 Yu Song;Zou Lianpeng;Zhao Jiawei;Zhu Yiping;Xue Liming;Lu Dasheng(Shanghai Institutes of Preventive Medicine,Shanghai 200336)

机构地区：[1]上海市预防医学研究院,上海200336

出　　处：《中国食品学报》2024年第4期99-107,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：上海市卫生健康委员会“卫生健康青年人才计划”项目(2022YQ054);上海市科学技术委员会“扬帆计划”项目(23YF1441900)。

摘　　要：交链孢毒素是一类广泛存在的食品污染物,交链孢酚(AOH)、交链孢酚单甲醚(AME)、细交链孢菌酮酸(TeA)、腾毒素(TEN)经常同时出现,然而目前对其联合毒性的评估十分有限。目的:以BRL-3A细胞为研究模型,评估常见4种交链孢毒素对肝脏细胞的联合毒性。方法:应用CCK-8试剂盒检测10~200μmol/L交链孢毒素单独或联合处理BRL-3A细胞24,48,72 h细胞活性的变化。基于组合指数法,分析它们混合暴露后的联合毒性效应。结果:AOH、AME、TeA和TEN单独处理BRL-3A细胞后,能够降低细胞存活率,并呈现剂量和时间依赖性,其IC50分别为38.11,41.25,49.05,358.58μmol/L(48 h)。AOH-AME、AME-TEN和TeA-TEN组合的毒性在IC_(10)~IC_(90)水平上都表现出累加或协同效应。AOH-AME-TeA、AME-TeATEN和AOH-AME-TeA-TEN组合的毒性在低剂量时具有拮抗效应,在高剂量时具有协同效应,而其它组合与之相反。结论:食品中常见的4种交链孢毒素均能不同程度地抑制BRL-3A细胞的增殖,具有肝脏细胞毒性,其毒性强度顺序为AOH>AME>TeA>>TEN。当它们同时出现时,其毒性作用可能为协同效应,提示今后应重点关注毒素的联合毒性风险,以更精准地评估其安全风险。Alternaria toxins are widespread food contaminants,accompanying with alternariol(AOH),alternariol monomethyl ether(AME),tenuazonic acid(TeA),and Tentoxin(TEN).Currently,there is limited assessment on their combined toxicity.Objective:To evaluate the combined toxicity of four common alternaria toxins on liver cells using BRL-3A cells as a research model.Methods:A CCK-8 kit was applied to detect changes in cell viability of BRL-3A cells treated with 10-200μmol/L alternaria toxin alone or in combination for 24,48,72 h.Based on the combination index method,the combined toxicity after their mixed exposure were analyzed.Results:When the BRL-3A cells were treated with AOH,AME,TeA and TEN alone,the cell survival rate was reduced in a dose-and time-dependent manner.Their IC50 were 38.11,41.25,49.05,358.58μmol/L(48 h),respectively.Moreover,the toxicity of AOH-AME,AME-TEN and TeA-TEN combinations all showed additive or synergistic effects at IC_(10)-IC_(90) levels.The toxicity of the AOH-AME-TeA,AME-TeA-TEN and AOH-AME-TeA-TEN combination showed antagonistic effects at low doses and synergistic effects at high doses,while the other combinations showed the opposite results.Conclusion:All four alternaria toxins in food inhibit the proliferation of BRL-3A cells in varying degrees,with toxicity intensity being AOH>AME>TeA>>TEN.When coexist,a synergistic effect occurs in their toxicity effect,suggests that we need to focus on the combined toxicity risk of toxins in order to assess their safety risk more accurately in the future.

关 键 词：交链孢毒素 肝脏毒性 联合毒性 组合指数法 风险评估 

分 类 号：TS201.6[轻工技术与工程—食品科学]