灵芝孢子粉多糖对APAP肝损伤的保护作用研究  

Protect Effects of Ganoderma lucidum Spore Polysaccharide on Liver Injury Caused by Acetaminophen

作　　者：刘洋 韩忠明[2] 刘琳玲[1] 李金涛[1] 张楠 闫梅霞 LIU Yang;HAN Zhongming;LIU Linling;LI Jintao;ZHANG Nan;YAN Meixia(Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112,China;College of Traditional Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《食品工业科技》2024年第11期289-295,共7页Science and Technology of Food Industry

基　　金：吉林省科技发展计划项目(20210401101YY)。

摘　　要：目的:探讨灵芝孢子粉多糖(Ganoderma lucidum spore polysaccharide,GLSP)在对乙酰氨基酚(Aceta-minophen,APAP)肝损伤中的保护作用。方法:将50只小鼠随机分为五组,分别为空白组、模型组、阳性药物组、GLSP低剂量组和GLSP高剂量组,每组10只,各组按相应剂量连续14 d预给药后,使用APAP造模、取材,测定肝脏指数、血清指标(谷草转氨酶(glutamic oxalacetic transaminase,AST)、谷丙转氨酶(glutamic-pyruvic transaminase,ALT))及肝组织匀浆指标(谷胱甘肽(glutathione,GSH)、丙二醛(malonaldehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、Caspase-3、Bax和Bcl-xl)。结果:与空白组相比,模型组中ALT、AST、MDA及促凋亡基因Caspase-3、Bax均极显著升高(P<0.01),SOD、GSH含量极显著降低(P<0.01),HE染色结果显示APAP处理的小鼠肝脏出现典型的小叶中心性坏死;多糖剂量组与模型组相比ALT、AST极显著降低(P<0.01),多糖低剂量组SOD、GSH显著升高(P<0.05),MDA显著降低(P<0.05),在多糖高剂量组中,SOD、GSH极显著升高(P<0.01),MDA极显著降低(P<0.01),多糖组促凋亡基因Caspase-3、Bax极显著降低(P<0.01),且肝组织病理学观察中可见肝组织坏死面积减小。结论:灵芝孢子粉多糖对APAP肝损伤能够起到预保护作用,其作用机制可能与提高肝脏的抗氧化能力和抑制凋亡的发生有关。Objective:To investigate the protective effects of Ganoderma lucidum spore polysaccharide(GLSP)on liver injury caused by acetaminophen(APAP).Methods:Fifty mice were randomly divided into five groups:Blank group,model group,positive drug group,GLSP low-dose group and GLSP high-dose group,with 10 mice in each group.After each group was pre-administered according to the corresponding dose for 14 consecutive days,the liver index,serum indexes(glutamic oxalacetic transaminase(AST),glutamic-pyruvic transaminase(ALT))and liver tissue plasma indexes(glutathione(GSH),malonaldehyde(MDA),superoxide dismutase(SOD),Caspase-3,Bax and Bcl-xl)were determined.Results:Compared with the blank group,ALT,AST,MDA and proapoptotic genes Caspase-3 and Bax were very significantly increased in the model group(P<0.01).The contents of SOD and GSH decreased very significantly(P<0.01),and HE staining showed that APAP treated mice showed typical lobular central necrosis of liver.Compared with model group,ALT and AST were very significantly decreased in polysaccharide dose group(P<0.01),SOD and GSH were significantly increased(P<0.05)and MDA was significantly decreased in polysaccharide low-dose group(P<0.05),SOD and GSH were very significantly increased(P<0.01)and MDA was very significantly decreased in polysaccharide high-dose group(P<0.01),and pro-apoptotic genes Caspase-3 and Bax were very significantly decreased in polysaccharide group(P<0.01),and the liver histopathological observation showed that the necrotic area of liver tissue decreased.Conclusion:Ganoderma lucidum spore polysaccharide can preprotect APAP liver injury,and its mechanism may be related to improving the antioxidant capacity of liver and inhibiting the occurrence of apoptosis.

关 键 词：灵芝孢子粉 多糖 APAP 抗氧因子 凋亡基因 

分 类 号：TS201.4[轻工技术与工程—食品科学]