野生接骨木果含片的制备及其抗氧化、抗运动疲劳作用研究  

Preparation of Wild Elderberry Tablets and Their Antioxidant and Anti-exercise Fatigue Effects

作　　者：王江[1] 马彩珍[1] 李甜 霍彦雄 张亮亮 吴子健[3] 徐建国 WANG Jiang;MA Caizhen;LI Tian;HUO Yanxiong;ZHANG Liangliang;WU Zijian;XU Jianguo(School of Physical Education,Shanxi Normal University,Taiyuan 030031,China;School of Food Science,Shanxi Normal University,Taiyuan 030031,China;School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China)

机构地区：[1]山西师范大学体育学院,山西太原030031 [2]山西师范大学食品科学学院,山西太原030031 [3]天津商业大学生物技术与食品科学学院,天津300134

出　　处：《保鲜与加工》2024年第5期69-76,共8页Storage and Process

基　　金：山西省自然科学基金(202103021223256,202303021211133)。

摘　　要：为制备一款口感适宜且具有抗氧化和抗疲劳作用的接骨木果含片,以野生接骨木果粉为原料,通过响应面试验优化接骨木果含片制备工艺;以1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)阳离子自由基清除能力和铁离子还原抗氧化能力(FRAP)作为评价其抗氧化能力的指标;通过测定小鼠游泳时间,分析血糖、肝糖原、肌糖原、超氧化物歧化酶(SOD)活性、三磷酸腺苷(ATP)、尿素氮(BUN)和乳酸(LA)含量变化来评价其抗疲劳作用。结果表明:接骨木果粉添加量61%、木糖醇添加量8%和柠檬酸添加量5%时,接骨木果含片的感官评分达到90.32分,其花色苷含量为1.52 mg/g;接骨木果含片的DPPH自由基清除能力、ABTS阳离子自由基清除能力和FRAP值分别为73.83μg TE/g、43.50μg TE/g和16.27μmol Fe(Ⅱ)/g,表现出良好的抗氧化能力;接骨木果含片干预后,延长了小鼠的游泳时长,提高了小鼠的血糖、肝糖原、肌糖原、SOD和ATP的含量,降低了小鼠体内BUN和LA水平,表明接骨木果含片具有较高的抗疲劳能力。该研究为野生接骨木果的高值化利用、扩大其应用提供了理论依据。In order to prepare elderberry fruit tablets with suitable taste,antioxidant and anti-fatigue effects,with el⁃derberry powder as raw material,the preparation process of elderberry fruit tablets was optimized through response sur⁃face test.1,1-diphenyl-2-trinitrophenyl hydrazine(DPPH)radical scavenging activity,2,2’-azino-bis(3-ethylbenzo⁃thiazoline-6-sulfonic acid(ABTS)cation radical scavenging activity and ferric reducing antioxidant power(FRAP)re⁃ducing ability were used as indicators to evaluate its antioxidant capacity.The anti-fatigue ability of mice was evaluat⁃ed by measuring the swimming time and analyzing the level changes of blood glucose,liver glycogen,muscle glycogen,superoxide dismutase(SOD),adenosine triphosphate(ATP),urea nitrogen(BUN),and lactate(LA).The results showed that the sensory score and anthocyanins content of elderberry fruit tablets were 90.23 and 1.52 mg/g,respectively,when the addition concentration of elderberry powder,xylitol,and citric acid was 61%,8%and 5%,respectively.Meanwhile,the DPPH radical,ABTS cation radical scavening capacities,and FRAP values of elderberry fruit tablets were 73.83μg TE/g,43.50μg TE/g and 16.27μmol Fe(II)/g,respectively,exhibiting good antioxidant capacity.In addi⁃tion,the intervention of elderberry fruit tablets prolonged the swimming time of mice,increased the levels of blood glu⁃cose,liver glycogen and muscle glycogen,and enhanced the SOD and ATP content,as well as reduced the levels of BUN and LA,demonstrating good anti-fatigue effects.Which provides a theoretical basis for the high-value utilization and expanding the application of elderberry fruit.

关 键 词：野生接骨木果 含片 抗氧化活性 抗疲劳活性 

分 类 号：TS201[轻工技术与工程—食品科学]