基于胶体金-适配体的层析试纸条检测玉米中黄曲霉毒素B_(1)  

Detection of aflatoxin B_(1)in corn by chromatography strip based on colloidal gold aptamer

作　　者：王亚芳 任文洁 何保山[1] WANG Yafang;REN Wenjie;HE Baoshan(College of Food Science and Engineering,National Engineering Laboratory/Key Laboratory of Henan Province,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学粮油食品学院,国家工程实验室/河南省重点实验室,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2024年第2期106-113,共8页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南工业大学粮油食品学科国家工程实验室/省重点实验室开放课题基金资助项目(NC2021013);河南省高等学校重点科研项目(22A550004)。

摘　　要：黄曲霉毒素B_(1)(AFB_(1))因其剧毒性及在粮食中分布广泛而成为人类健康和粮食安全的重大风险因素之一,因此建立快速、灵敏的检测方法十分必要。利用胶体金和适配体作为金标探针,构建一种新型层析试纸条以实现玉米中AFB_(1)的快速检测。根据体系中AFB_(1)存在会减弱生物素标记的适配体与金标探针的结合,从而导致试纸条检测线的颜色强度降低或消失,进而实现对AFB_(1)高效灵敏检测。在最佳条件下,试纸条检测AFB_(1)的检出限值低至0.5 ng/mL,在0.5~500 ng/mL范围内快速准确检测且对AFB_(1)具有高度的特异性。本研究制备的适配体试纸条能够成为玉米中AFB_(1)检测的有效方法,具有良好的应用前景。Aflatoxin B_(1)(AFB_(1))has become one of the significant risk factors for human health and food security due to its hypertoxicity and wide distribution in grain.Therefore,it is necessary to detect it.In this study,a novel chromatographic test strip(AuNPs-LFAA)was constructed for the rapid detection of AFB_(1)in maize using colloidal gold(AuNPs)and aptamer as the nano-golden probe.Based on the fact that the presence of AFB_(1)in the system attenuates the binding of biotin-modified aptamers(Bio-Apt)and thiol-modified complementary chain covalently bound AuNPs(AuNPs-SHcDNA)will be weakened,thus the color intensity of the test line of the test strip would be reduced.Therefore,how to achieve efficient and sensitive detection of AFB_(1)is required.Under the optimal conditions,AuNPs-LFAA detected AFB_(1)with a limit of detection(LOD)as low as 0.5 ng/mL,providing a rapid and accurate detection in the range of 0.5-500 ng/mL with high specificity and sensitivity for AFB_(1).In summary,AuNPs-LFAA prepared in this study can be an effective method for the detection of AFB_(1)in maize and has good application prospects.

关 键 词：适配体 金纳米粒子 比色法 层析试纸条 黄曲霉毒素B_(1) 

分 类 号：TS207.3[轻工技术与工程—食品科学]