机构地区:[1]新疆医科大学第一附属医院/附属口腔医院口腔颌面肿瘤外科 [2]新疆维吾尔自治区口腔医学研究所,乌鲁木齐830054
出 处:《新疆医科大学学报》2024年第5期681-686,共6页Journal of Xinjiang Medical University
基 金:新疆维吾尔自治区区域协同创新专项项目(2020E02130)。
摘 要:目的采用微焦点断层扫描(Micro-CT)评价超声骨焊接技术应用过程中超声震荡产热作用下PDLLA材料对骨愈合性能的影响。方法选择SPF级雄性新西兰大白兔36只,随机分为超声骨焊接技术辅助PDLLA材料组(A组)、拧入技术辅助PDLLA材料组(B组)、假手术组(C组)、空白对照组(D组),各9只。于术后4、8、12周取下颌骨标本,周围骨组织进行HE染色,观察各组植入钉周围情况。对术后4、8、12周的下颌骨大体标本拍摄Micro-CT图,使用VG Studio MAX软件进行三维重建,摆正数据样本,即颌骨颊侧造模处为轴向,动态分析植入钉体积、植入钉周围200μm环状区及骨缺损处的植入材料体积数、相对骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数(Tb.N)、骨小梁间隙(Tb.Sp)等骨愈合相关指标。结果HE染色结果显示,按3个时间可动态观察到A、B、C组骨缺损处骨性骨痂替代纤维性骨痂,形成类骨质,编织骨的过程符合正常骨损伤愈合的过程。Micro-CT结果显示,A、B组术后3个时间点的材料体积比较差异均无统计学意义(P>0.05);在3个时间点,4组植入钉周围200μm环状区中BV/TV、Tb.Th、Tb.N、Tb.Sp比较差异均无统计学意义(P>0.05);在骨缺损处,A、B、C组三个时间点BV/TV、Tb.Th、Tb.N、Tb.Sp比较差异无统计学意义(P>0.05);术后4周,与D组比较,A、B、C组BV/TV、Tb.Th、Tb.N、Tb.Sp差异有统计学意义(P<0.05);与C组比较,A、B组Tb.N差异有统计学意义(P<0.01)。术后8周,与D组比较,A、B、C组BV/TV、Tb.Th、Tb.N、Tb.Sp差异有统计学意义(P<0.05);术后12周,与D组比较,A、B、C组BV/TV、Tb.Th、Tb.Sp差异有统计学意义(P<0.05)。结论超声骨焊接技术其超声震荡产热作用下PDLLA材料对骨愈合性能无不良影响。Objective To evaluate the influence of ultrasonic vibration heating produced by ultrasonic bone welding technology on the bone healing performance of PDLLA material during the application process u-sing micro-computed tomography(Micro-CT).Methods 36 male New Zealand white rabbits were selected and randomly divided into 4 groups:ultrasonic bone welding technique assisted PDLLA material group(A group),Screw-in technical auxiliary PDLLA material group(B group),sham group(C group),and blank control group(D group),with 9 rabbits in each group.After 4,8 and 12 weeks of the surgery,the mandibular bone specimens were removed,and the surrounding bone tissue was stained with hematoxylin and eosin(HE)to observe the surrounding conditions of the implanted pins in each group.Micro-CT images of the mandibular bone specimens were taken at 4,8 and 12 weeks postoperatively,and the data were reconstructed in 3 dimensions using VG Studio MAX software.The data samples were aligned,with the buccal side of the mandible as the axial direction,and the dynamic analysis of the number of volume of the implanted material,the relative bone volume(BV/TV),trabecular thickness(Tb.Th),trabecular number(Tb.N)and trabecular spacing(Tb.Sp)and other bone healing-related indicators was conducted.Results The results of HE staining showed that according to 3 times,bone callus can be observed in groups A,B and C to form bone.The process of woven bone was in line with the process of normal bone injury healing.The micro-CT results showed that there was no significant comparison of material volume difference between the 3 postoperative time points in groups A and B(P>0.05);At the 3 time points,No significant comparison of BV/TV,Tb.Th,Tb.N and Tb.Sp results in the 200μm ring area around the 4 groups(P>0.05);In the bone defect area,there was no significant difference between BV/TV,Tb.Th,Tb.N and Tb.Sp in groups A,B and C(P>0.05).There were significant differences in BV/TV,Tb.Th,Tb.N and Tb.Sp in groups A,B and C(P<0.05).Compared with group C,the difference
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