马蹄三部位生物活性初筛  

Preliminary Screening of Biological Activity of Three Parts of Water Chestnut

作　　者：高紫珊 杨意 李军 谢镇蔚 萧雅泳 敬思群 华军利[1] 康会茹 肖志平 杨柳斌 GAO Zishan;YANG Yi;LI Jun;XIE Zhenwei;XIAO Yayong;JING Siqun;HUA Junli;KANG Huiru;XIAO Zhiping;YANG Liubin(School of Food Science&Technology,Shaoguan University,Shaoguan 512005,China;Lechang City Beixiang Guiyuan Water Chestnut Circulation Professional Cooperative,Lechang City,Lechang 512200,China;College of Biology and Agriculture,Shaoguan University,Shaoguan 512005,China)

机构地区：[1]韶关学院食品学院,广东韶关512005 [2]乐昌市北乡桂源马蹄流通专业合作社,广东乐昌512200 [3]韶关学院生物与农业学院,广东韶关512005

出　　处：《中国果菜》2024年第5期40-47,共8页China Fruit & Vegetable

基　　金：广东省驻镇帮镇扶村农村科技特派员项目(第2批)(KTP20210164);韶关学院2022年度大学生创新创业训练计划(202210576018);2024年度广东省科技创新战略专项资金(大学生科技创新培育)重点项目(pdjh2024a336);2023年广东省普通高校重点领域专项(科技服务乡村振兴)资金资助(2023ZDZX4054);韶关学院“双百行动”专项。

摘　　要：为探究马蹄地上茎、皮、球茎三部位的生物活性,采用超声辅助提取法得到马蹄三部位的水提物和醇提物,分别评价其抗氧化、抑菌和体外降糖活性;同时利用果蝇II型糖尿病模型,考察马蹄三部位原料的体内降糖降脂功效。结果表明,马蹄三部位提取物都具有DPPH、ABTS+自由基清除能力和α-葡萄糖苷酶、α-淀粉酶抑制能力,且都与浓度呈一定剂量关系,马蹄皮水提物的抗氧化效果最强,马蹄地上茎水提物体外降糖作用最强;除黑曲霉外,马蹄三部位提取物对其他三种种菌都有一定的抑制作用,尤其对金黄色葡萄球菌抑菌作用最强;马蹄三部位均能降低果蝇体内的海藻糖、甘油三酯含量,且对总蛋白含量无显著影响,以马蹄皮降糖功效最强、马蹄球茎降脂作用最强。The purpose of this paper is reveal the biological activity of its aboveground stem,its pericarp and bulb of the three parts form water chestnut.The ultrasonic assisted extraction method was used to obtain the water extract and alcohol extract of three parts of Eleocharis dulcis.With its ability to eliminate DPPH,ABTS+free radicals,its inhibition zone and minimum inhibitory concentration against Staphylococcus aureus,Escherichia coli,Saccharomyces cerevisiae and Aspergillus niger,and its inhibitory effect onα-glucosidase andα-amylase was evaluated for its antioxidant,antibacterial,and in vitro hypoglycemic activities;At the same time,we used Drosophila type II diabetes model to investigate the hypoglycemic and lipid-lowering effects in vivo of three parts of water chestnut.The results showed that the extracts of three parts of E.dulcis all had DPPH,ABTS+radical scavenging ability,and the inhibitory ability ofα-glucosidase,α-amylase with a dose-effect relationship,and the water extract of pericarp from water chestnut has the strongest antioxidant effect while the water extract of its aboveground stem had the strongest hypoglycemic effect in vitro.In addition to A.niger,the extract from three parts of water chestnut had certain antibacterial effect on the other three kinds of bacteria,especially on S.aureus.The extracts from all three parts of water chestnut could reduce the contents of trehalose and triglyceride in Drosophila melanogaster,and had no significant effect on the contents of total protein while the water chestnu pericarp had the strongest hypoglycemic effect,and bulb of water chestnut had the strongest hypolipic effect.

关 键 词：马蹄三部位 抗氧化性 抑菌性 果蝇II型糖尿病模型 降糖降脂作用 

分 类 号：S682.264[农业科学—观赏园艺]