Photochemically induced thalamus infarction impairs cognition in a mouse model  

作　　者：Chen Zhang Shiping Li Yongjun Wang Jiong Shi 

机构地区：[1]Department of Neurology,Beijing Tiantan Hospital,Capital Medical University,Beijing,China [2]China National Clinical Research Center for Neurological Diseases,Beijing,China [3]Advanced Innovation Center for Human Brain Protection,Capital Medical University,Beijing,China

出　　处：《Stroke & Vascular Neurology》2023年第6期444-452,I0001,I0002,共11页卒中与血管神经病学（英文）

基　　金：the Beijing Municipal Science&Technology Commission(Grant No.D171100003017002);National Key R&D Program of China(Grant No.2018YFC1312903);Strategic Priority Research Program of the Chinese Academy of Sciences(XDB39000000);National Science and Technology Major Project(Grant No.2017ZX09304018).

摘　　要：Background Small subcortical infarcts account for up to 25%of ischaemic strokes.Thalamus is one of the subcortical structures that commonly manifest with lacunar infarcts on MRI of the brain.Studies have shown that thalamus infarction is associated with cognitive decline.However,due to the lack of proper animal models,little is known about the mechanism.We aimed to establish a focal thalamus infarction model,characterise the infarct lesion and assess functional effects.Methods Male C57BL/6J mice were anaesthetised,and Rose Bengal dye was injected through the tail vein.The right thalamus was illuminated with green laser light by stereotactic implantation of optic fibre.Characteristics of the infarct and lesion evolution were evaluated by histological analysis and 7T MRI at various times.The cognitive and neurological functions were assessed by behavioural tests.Retrograde tracing was performed to analyse neural connections.Results An ischaemic lesion with small vessel occlusion was observed in the thalamus.It became a small circumscribed infarct with reactive astrocytes accumulated in the infarct periphery on day 21.The mice with thalamic infarction demonstrated impaired learning and memory without significant neurological deficits.Retrogradely labelled neurons in the retrosplenial granular cortex were reduced.Conclusion This study established a mouse model of thalamic lacunar infarction that exhibits cognitive impairment.Neural connection dysfunctions may play a potential role in post-stroke cognitive impairment.This model helps to clarify the pathophysiology of post-stroke cognitive impairment and to develop potential therapies.

关 键 词：INFARCTION THALAMUS IMPAIRED 

分 类 号：R743.3[医药卫生—神经病学与精神病学]