裙带菜孢子叶组分及其生物活性研究  

Study on Components and Biological Activity from Sporophyll of Undaria Pinnatifida

作　　者：张洋 ZHANG Yang(Wuhu Institute of Technology,Anhui Wuhu 241003,China)

机构地区：[1]芜湖职业技术学院,安徽芜湖241003

出　　处：《广州化工》2024年第1期60-63,共4页GuangZhou Chemical Industry

基　　金：安徽省高校自然科学研究重点项目(KJ2020A0921)。

摘　　要：裙带菜(Undaria pinnatifida)含有丰富的营养物质,具有增强免疫力、抗肿瘤、抗凝血等作用,其孢子叶中具有多种生物活性物质,为研究裙带菜孢子叶在健康食品及化妆品领域的应用,从裙带菜孢子叶中提取活性物质,并对其成分进行了分析。提取收率分别为色素7.0%、褐藻糖胶3.6%、海藻酸钠5.9%。硫酸苯酚法测定孢子叶中糖含量为13.5%、福林酚法测定多酚含量为19.32 mg GAE/g、二喹啉甲酸法测定蛋白质含量为9.92%。IR表明孢子叶中提取的多糖、海藻盐分别为褐藻糖胶和海藻酸钠。裙带菜孢子叶中提取的褐藻糖胶DPPH自由基清除活性407.9μmol TE/g,透明质酸酶抑制活性82%,此研究为裙带菜孢子叶中提取的褐藻糖胶、海藻酸钠作为保健食品、药品和化妆品的开发利用提供理论依据。Undaria pinnatifida possesses abundant nutritional components exert immuno-boosting,anti-tumor,and anticoagulant effects.Its sporophylls contains a variety of bioactive substances,inspiring research into their potential applications in health foods and cosmetics.Investigators have extracted active substances from the sporophylls of Undaria pinnatifida and analyzed their components to further explore these applications.The extraction yields were as follows:7.0% pigment,3.6%fucoidan,and 5.9% sodium alginate.Upon determination,the sugar content in the sporophyll was 13.5% by phenol-sulfuric acid method,the polyphenol content was 19.32 mg GAE/g by Folin Phenol method and the protein content was 9.92% by BCA.IR revealed that the extracted polysaccharides and alginic acid salts in the sporophyll were fucoidan and sodium alginate,respectively.The DPPH free radical scavenging activity of the fucoidan extracted from undaria pinnatifida sporophyll was 407.9μmol TE/g,and its hyaluronidase-inhibitory activity reached 82%.This study served as a theoretical basis for the development and utilization of fucoidan and sodium alginate extracted from the sporophylls of Undaria pinnatifida as health foods,pharmaceuticals and cosmetics.

关 键 词：裙带菜 孢子叶 褐藻糖胶 DPPH 透明质酸酶 

分 类 号：S986.2[农业科学—捕捞与储运]