蜜环菌菌株M6的液体深层发酵工艺研究  

Research on Liquid Submerged Fermentation Process of Armillaria mellea M6 Strain

作　　者：罗成莹 罗智文 刘佳佳 林连兵[1,2] LUO Cheng-ying;LUO Zhi-wen;LIU Jia-jia;LIN Lian-bing(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]云南省高校饲用抗生素替代技术工程研究中心,云南昆明650500

出　　处：《中药材》2024年第1期22-26,共5页Journal of Chinese Medicinal Materials

基　　金：云南省重大科技资助项目(202202AG050008)。

摘　　要：目的:优化蜜环菌菌株M6的发酵工艺及提高其生产效率。方法:以蜜环菌菌株M6为供试菌株,通过摇瓶液体培养,筛选该菌株的最适液体发酵培养基;以摇瓶培养结果为基础,通过多级匀浆制备高活性种子液,进行液体深层发酵,优化发酵条件。结果:筛选出蜜环菌菌株M6最佳液体深层发酵条件为转速150 r/min、接种量5%、溶氧20%及温度28℃,在此条件下使用液体发酵培养基配方1发酵180 h所得蜜环菌菌株M6生物量干重高达18.39 g/L;使用液体发酵培养基2发酵120 h所得蜜环菌菌株M6生物量干重达23.83 g/L。结论:液体培养条件优化后,蜜环菌菌株M6生物量产出较高。本研究促进了蜜环菌菌株M6的快速生产,为应用于天麻种植业提供了技术参考。Objective:To optimize the fermentation process and improve its production efficiency of Armillaria mellea M6 strain.Methods:With Armillaria mellea M6 strain as the test strain,the optimum liquid fermentation medium of the strain was screened by shaking bottle liquid culture.Based on the results of shaker culture,the highly active seed liquid was prepared by multistage homogenization,and the liquid deep fermentation was carried out to optimize the fermentation conditions.Results:The optimal liquid deep fermentation conditions for Armillaria mellea M6 strain were as follows:rotational speed 150 r/min,inoculation volume 5%,dissolved oxygen 20%and temperature 28℃.Under these conditions,the biomass dry weight of Armillaria mellea M6 strain was 18.39 g/L after fermentation for 180 hours using liquid fermentation medium formula 1.Using liquid fermentation medium 2 for 120 hours,the biomass dry weight of Armillaria mellea M6 strain was 23.83 g/L.Conclusion:After the optimization of liquid culture conditions,the biomass production of Armillaria mellea M6 strain is higher.This study promotes the rapid production of Armillaria mellea M6 strainand provides a technical reference for its application in Gastrodia elata cultivation.

关 键 词：蜜环菌 培养基 发酵工艺优化 液体深层发酵 

分 类 号：Q815[生物学—生物工程]