双波长叠加可见分光光度法测定天然甜味剂甘草酸的含量  

Determination of the content of natural sweetener glycyrrhizinic acid by dual wavelength superimposed visible spectrophotometry

作　　者：储文 黄佃宇 田美 马卫兴 CHU Wen;HUANG Dianyu;TIAN Mei;MA Weixing(School of Pharmacy,Jiangsu Ocean University,Lianyungang 222005;Biomedical Industry College,Jiangsu Ocean University,Lianyungang 222005;Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening,Lianyungang 222005)

机构地区：[1]江苏海洋大学药学院,连云港222005 [2]江苏海洋大学生物医药产业学院,连云港222005 [3]江苏省海洋药物活性分子筛选重点实验室,连云港222005

出　　处：《中国食品添加剂》2024年第5期287-294,共8页China Food Additives

基　　金：2021年江苏省首批一流本科课程(线下一流课程-药物分析,序号104)。

摘　　要：由于甘草酸二铵的稳定性和生物活性优于甘草酸,采用测定甘草酸二铵含量来间接测定甘草酸的含量,建立一种新的甘草酸的双波长叠加分光光度的测定方法。在pH 3.8的Clark-Lubs介质中,甘草酸二铵以甘草酸形式与结晶紫反应形成以氢键组装的超分子聚集体,最大负吸收波长位于496 nm,次大正吸收波长位于594 nm。甘草酸二铵的质量浓度与吸光度差值ΔA在一定范围内均呈良好的线性关系。采用双波长叠加法测定时,线性范围是1.8~4.2 mg/L,相关系数为0.9989,表观摩尔吸光系数可达5.85×105 L/(mol·cm),检出限为0.098 mg/L。应用此方法测定了市售不同级别酱油样品中甘草酸的含量,测定值的相对标准偏差(n=6)均在2%以内。该方法操作简便,灵敏度高,可用于酱油中甘草酸的快速测定。Since the stability and biological activity of diammonium glycyrrhizinate is better than those of glycyrrhizin acid,a new dual wavelength spectrophotometry method for the determination of glycyrrhetinic acid was established by indirectly measuring the content of diammonium glycyrrhetinic acid.In the Clark-Lubs medium at pH 3.8,diammonium glycyrrhizinate in the form of glycyrrhizic acid reacted with crystal violet to assemble a supramolecular agglomeration with hydrogen bonds,its maximum negative absorption wavelength was at 496 nm and the second largest positive absorption wavelength was at 594 nm.The mass concentration of diammonium glycyrrhizinate and the absorbance differenceΔA showed a good linear relationship within a certain range.When glycyrrhizinic acid was detected by dual wavelength superposition method,the linear range was 1.8~4.2 mg/L,correlation coefficient was 0.9989,the molar absorbance coefficient could reach 5.85×105 L/(mol·cm),and the detection limit was 0.098 mg/L.The content of glycyrrhizinic acid in different commercially soy sauce samples was determined;and the relative standard deviation(n=6)of the measured values was within 2%.This method was easy to operate,had high sensitivity.It can be used to rapidly determine glycyrrhizinic acid in soy sauce.

关 键 词：甘草酸 结晶紫 可见分光光度法 双波长 酱油 甜味剂 

分 类 号：TS207.3[轻工技术与工程—食品科学]