基于UPLC-QE-MS对关中奶山羊泌乳盛期和泌乳末期生乳差异的分析  

UPLC-QE-MS Analysis of the Differences in Milk Metabolites from Guanzhong Dairy Goats at the Peak and Late Lactation Stages

作　　者：余梦琦 袁宇欣 陈璐 沙日阿康 任新阳 屈颖新 李广[1] YU Mengqi;YUAN Yuxin;CHEN Lu;SHARI Akang;REN Xinyang;QU Yingxin;LI Guang(College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《食品科学》2024年第10期186-193,共8页Food Science

基　　金：陕西省农业协同创新与推广联盟项目(LMZD202002);陕西省农业科技创新推广项目(NYKJ-2019-YL16)。

摘　　要：为探究山羊乳中代谢物以及代谢物在不同泌乳期的动态变化和阶段差异性,采用超高效液相色谱-四极杆静电场轨道阱质谱法比较关中奶山羊泌乳盛期和泌乳末期生乳中的差异代谢产物。对表征的代谢物以P<0.05、变量投影重要度(variableimportanceintheprojection,VIP)>1.00、差异倍数(foldchange,FC)>2或FC<0.5为筛选标准,共筛选出90种差异代谢物。与泌乳末期相比,泌乳盛期的山羊乳中有50种差异代谢物上调,40种下调。代谢途径分析表明,这些差异代谢物与次级胆汁酸生物合成显著相关。根据VIP值,尿苷二磷酸-N-乙酰基葡糖胺、磷脂酰乙醇胺(12:0/22:2(13Z,16Z))可考虑作为关中奶山羊两个时期差异变化的潜在生物标志物,但还待进一步的研究验证。In order to explore the dynamic changes of metabolites in goat milk during different lactation periods,the differential metabolites in milk from Guanzhong dairy goats at the peak and late lactation stages were investigated by ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry(UPLC-QE-MS).A total of 90 differential metabolites were identified using P<0.05,variable importance in the projection(VIP)>1.00,and fold change(FC)>2 or<0.5 as criteria.Compared with late-lactation milk,50 differential metabolites were up-regulated and 40 down-regulated in peak-lactation milk.Metabolic pathway analysis showed that these differential metabolites were significantly correlated with the biosynthesis of secondary bile acids.According to the VIP,uridine diphosphate-N-acetylglucosamine and phosphatidylethanolamine(PE,12:0/22:2(13Z,16Z))could be considered as potential biomarkers for the differential changes of milk metabolites from Guanzhong dairy goats during the two lactation periods,but this needs further research to be confirmed.

关 键 词：山羊乳 泌乳盛期 泌乳末期 代谢组学 

分 类 号：TS252.2[轻工技术与工程—农产品加工及贮藏工程]