短小芽孢杆菌(GXUN613L)对银鲑生长、血清生化指标、抗氧化能力及肠道健康的影响  

Effects of Bacillus pumilus(GXUN613L)on growth,serum biochemical indexes,antioxidant capacity,and intestinal health of coho salmon(Oncorhynchus kisutch W.)

作　　者：梁俊杰 杨秋月 许剑 刘永强 童潼 王佳婧 徐粒潇 张琴 LIANG Jun-jie;YANG Qiu-yue;XU Jian;LIU Yong-qiang;TONG Tong;WANG Jia-jing;XU Li-xiao;ZHANG Qin

机构地区：[1]广西民族大学海洋与生物技术学院广西多糖材料与改性重点实验室广西民族大学海洋生物资源保护与利用重点实验室,广西南宁530006

出　　处：《饲料研究》2024年第8期66-71,共6页Feed Research

基　　金：广西创新驱动发展专项资金项目(项目编号:桂科AA17204044);广西民族大学引进人才科研启动项目(项目编号:2018KJQD14);广西民族大学相思湖青年学者创新团队资助项目(项目编号:2018RSCXSHQN02);大学生创新创业训练计划(项目编号:S202110608106);山东省重大科技创新工程项目(项目编号:2019JZZY020710)。

摘　　要：试验旨在探讨短小芽孢杆菌(GXUN613L)对银鲑生长性能、血清生化指标、抗氧化能力及肠道健康的影响。挑选240尾初始体质量为(130.2±10.1) g的银鲑,随机分为4组,每组3个重复,每个重复20尾鱼。CK组、LL组、LM组和LH组分别在基础饲料中添加0、2×10^(7)、4×10^(9)、8×10^(11) CFU/g的短小芽孢杆菌(GXUN613L)。试验期6 w。结果显示,LL组和LM组银鲑的增重率、特定生长率显著高于对照组(P<0.05);LM组银鲑的蛋白质效率显著高于对照组(P<0.05),饲料系数显著低于CK组(P<0.05)。LL组、LM组和LH组银鲑血清中的总蛋白、白蛋白和总胆固醇含量均显著高于CK组(P<0.05),LM组银鲑血清中葡萄糖含量和碱性磷酸酶活性显著高于CK组(P<0.05)。LM组银鲑肝脏的总抗氧化能力、过氧化氢酶、超氧化物歧化酶及谷胱甘肽过氧化物酶活性显著高于CK组(P<0.05)。LM组银鲑前肠、后肠肌层厚度及绒毛高度显著高于CK组(P<0.05),肠道微生物多样性更好。研究表明,饲料中添加短小芽孢杆菌(GXUN613L)可以促进银鲑生长,改善银鲑血清生化指标及肠道健康,提高银鲑的抗氧化能力,其适宜添加量为4×109 CFU/g。The purpose of this experiment was to investigate the effects of Bacilluspumilus(GXUN613L)on the growth performance,serum biochemical indexes,antioxidant capacity,and intestinal health of coho salmon.A total of 240 coho salmon with initial body mass of(130.2±10.1)g were randomly divided into four groups with three replicates in each group and 20 coho salmon in each replicate.Bacilluspumilus(GXUN613L)was supplemented with 0,2×10^(7),4×10^(9),and 8×10^(11) CFU/g in basal diet in CK group,LL group,LM group,and LH group,respectively.The trial period was six weeks.The results showed that the weight gain rate and specific growth rate of coho salmon in LL group and LM group were significantly higher than those in CK group(P<0.05),the protein efficiency ratio of coho salmon in LM group was significantly higher than that in CK group(P<0.05),and the feed conversion ratio was significantly lower than that in CK group(P<0.05).Serum total protein,albumin and total cholesterol in LL group,LM group,and LH group were significantly higher than those in CK group(P<0.05).Serum glucose content and alkaline phosphatase activity in LM group were significantly higher than those in CK group(P<0.05).The total antioxidant capacity,catalase activity,superoxide dismutase,and glutathione peroxidase activity of liver in LM group were significantly higher than those in CK group(P<0.05).The muscular layer thickness and villus height of foregut and hindgut in LM group were significantly higher than those in CK group(P<0.05),and the intestinal microbial diversity was better.The result shows that the addition of Bacilluspumilus(GXUN613 L)in feed can promote the growth,improve the serum indexes and intestinal health,and enhance the antioxidant capacity of coho salmon.The suitable addition amount of GXUN613L is 4×10^(9) CFU/g.

关 键 词：短小芽孢杆菌 银鲑 生长性能 血清生化指标 抗氧化能力 肠道健康 

分 类 号：S963.73[农业科学—水产养殖]