基于差分脉冲伏安法快速检测花椒油树脂中的麻味物质  

Rapid Detection of Numb-Taste Substances in Zanthoxylum bungeanum Oleoresin Based on Differential Pulse Voltammetry

作　　者：白春晖 蒋茜[1] 赵志峰[1] 谷学权 幸勇 莫小燕 何强[1] BAI Chun-hui;JIANG Xi;ZHAO Zhi-feng;GU Xue-quan;XING Yong;MO Xiao-yan;HE Qiang(College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,China;Sichuan Wufeng Lihong Food Co.,Ltd.,Ya'an 625302,China)

机构地区：[1]四川大学轻工科学与工程学院,成都610065 [2]四川五丰黎红食品有限公司,四川雅安625302

出　　处：《中国调味品》2024年第6期162-167,共6页China Condiment

摘　　要：麻味物质含量是花椒油树脂的重要品质指标,文章基于电化学原理中的差分脉冲伏安法首次建立了一种采用未修饰的丝网印刷电极测定花椒油树脂中麻味物质的快速检测方法,通过优化电化学工作站中的富集时间、电压以及待测液中的甲醇占比、pH值,并以紫外分光光度计检测法为对照构建出了该方法。结果表明,以未修饰的丝网印刷电极检测花椒油树脂中麻味物质的适宜条件:富集时间为120 s,富集电压为0.2 V,甲醇在待测液中占比小于5%,pH值为1,2,3,山椒素的电流值与其浓度在2~50 mg/L范围内呈良好的线性关系(R^(2)=0.999),加标回收率在97.60%~108.40%之间,检出限为0.22 mg/L。经验证,该方法与行业标准GH/T 1290—2020方法具有良好的检测一致性(RSD=4.75%),建立的快速检测方法既具有操作简单、方便快速的优点,又兼具传统方法的准确性与重复性,因此可以作为花椒及其制品中麻味物质的快速检测方法进行科学研究与企业生产实践,且有望基于该方法开发出便携式麻味物质快速检测仪器。The content of numb-taste substances is an important quality index of Zanthoxylum bungeanum oleoresin.Based on differential pulse voltammetry in electrochemistry principles,a rapid detection method for the determination of numb-taste substances in Zanthoxylum bungeanum oleoresin with unmodified screen-printed electrode is established for the first time.By optimizing the enrichment time,voltage in the electrochemical workstation,the proportion of methanol in the tested solution and pH value,the method is constructed with ultraviolet spectrophotometer detection method as the control.The results show that the optimum conditions for the detection of numb-taste substances in Zanthoxylum bungeanum oleoresin with unmodified screen-printed electrode are as follows:enrichment time is 120 s,enrichment voltage is 0.2 V,the proportion of methanol in the tested solution is less than 5%and pH value is 1,2,3,the current value of sanshool shows a good linear relationship with its concentration in the range of 2~50 mg/L(R^(2)=0.999),the spiked recovery rates are 97.60%~108.40%and the limit of detection is 0.22 mg/L.It is verified that this method has good detection consistency with the industry standard GH/T 1290—2020 method(RSD=4.75%).The established rapid detection method not only has the advantages of simple,convenient and fast operation,but also has the accuracy and repeatability of the traditional method.Therefore,it can be used as a rapid detection method of numb-taste substances in Zanthoxylum bungeanum and its products for scientific research and enterprise production practice,and it is expected to develop a portable rapid detection instrument of numb-taste substances based on this method.

关 键 词：花椒 山椒素 差分脉冲伏安法(DPV) 丝网印刷电极 紫外分光光度法 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]