广叶绣球菌多糖磷酸化修饰及体外抗氧化和降糖活性  

Phosphorylation of Sparassis latifolia Polysaccharides and Their Antioxidant and Hypoglycemic Activities In vitro

作　　者：崔芳铭 毕书瑜 侯江燕 曹谨玲 程艳芬[1,2] 程菲儿 云少君[1,2] 冯翠萍 CUI Fangming;BI Shuyu;HOU Jiangyan;CAO Jinling;CHENG Yanfen;CHENG Feier;YUN Shaojun;FENG Cuiping(College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,Shanxi,China;Shanxi Key Laboratory of Edible Fungi for Loess Plateau,Taigu 030801,Shanxi,China)

机构地区：[1]山西农业大学食品科学与工程学院,山西晋中030801 [2]黄土高原食用菌山西省重点实验室,山西晋中030801

出　　处：《食用菌学报》2024年第3期51-58,共8页Acta Edulis Fungi

基　　金：山西省现代农业产业技术体系建设项目(2023CYJSTX09);山西省基础研究计划项目(202103021224126);食用菌山西省科技创新重点团队(201805D131009)。

摘　　要：对广叶绣球菌(Sparassis latifolia)多糖(SLPs)进行磷酸化修饰,表征磷酸化SLPs(P-SLPs)结构,分析其体外抗氧化、降糖活性。结果表明:与SLPs比较,P-SLPs的磷酸根质量分数较高,为(8.02±0.48)%;在相同质量浓度时,P-SLPs对DPPH、超氧阴离子、羟基自由基和H_(2)O_(2)的清除率均较高,对α-淀粉酶和α-葡萄糖苷酶的抑制率均较高。研究结果可为广叶绣球菌多糖进一步开发应用提供参考。Sparassis latifolia polysaccharides(SLPs) were modified by phosphorylation,and then characterized for structure,and analyzed for antioxidant and hypoglycemic activities in vitro.The results showed that compared with SLPs,phosphorylated SLPs(P-SLPs) had a higher mass fraction of phosphate ions,which was(8.02±0.48)%.Under the same mass concentration,P-SLPs showed higher abilities on scavenging DPPH radicals,superoxide anion radicals,hydroxyl radicals,and H_2O_(2) than SLPs.The inhibitory activities of P-SLPs on α-amylase and α-glucosidase were also significantly promoted compared with SLPs.The research results provided a reference for further development and application of SLPs.

关 键 词：广叶绣球菌多糖 磷酸化修饰 抗氧化 降糖活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]