四类发酵助剂对红酵母产类胡萝卜素的影响  

The Effect of Four Types of Fermentation Aids on the Production of Carotenoids by Rhodotorula

作　　者：何宇光 罗正阳 李达[1] 苗欣宇 孙慕白 牛红红[1] 华梅 苏颖[1] 王景会[1] HE Yuguang;LUO Zhengyang;LI Da;MIAO Xinyu;SUN Mubai;NIU Honghong;HUA Mei;SU Ying;WANG Jinghui(Agricultural Product Processing Research Institute,Jilin Academy of Agricultural Sciences,Changchun,Jilin 130033,China)

机构地区：[1]吉林省农业科学院农产品加工研究所,吉林长春130033

出　　处：《农产品加工》2024年第10期15-19,共5页Farm Products Processing

基　　金：吉林省科技发展计划重点研发项目“红酵母JAASR固态发酵研究及其在蛋鸡饲养中的应用”(202202051NC)。

摘　　要：为了提高红酵母JAASSRY(Rhodotorula.glutinis JAASSRY)类胡萝卜素的合成能力,通过在发酵过程中添加4种不同类型的发酵助剂,以类胡萝卜素含量和菌体生物量为标准,探究最佳发酵助剂的种类及添加量。结果表明,适量添加发酵助剂能够有效提升红酵母的类胡萝卜素合成能力,其中维生素B族的维B_(1)(2.8 mg/L)和油脂类的大豆油(0.6 mL/L)是最适宜的发酵助剂,2种助剂的类胡萝卜素产量分别达到了224.01μg/g和325.85μg/g,生物量分别为1.30 g/L和0.90 g/L,并且在提升合成能力的同时还具有成本低、稳定性高的特点。添加维B_(1)(2.8 mg/L)和大豆油(0.6 mL/L)能够显著提升红酵母JAASSRY菌株的类胡萝卜素合成能力,是较为理想的红酵母发酵助剂。In order to improve the carotenoid synthesis ability of Rhodotorula.glutinis(JAASSRY),four different types of fermentation aids were added during the fermentation process.Based on the carotenoid content and bacterial biomass,the optimal type and aids were explored.The results showed that adding an appropriate amount of fermentation aids could effectively enhance the carotenoid synthesis ability.Among them,VB_(1)(2.8 mg/L)of Vitamin B group and soybean oil(0.6 mL/L)of oils were the most suitable fermentation aids,and the carotenoid production of the two aids reached 224.01μg/g and 325.85μg/g,biomass was 1.30 g/L and 0.90 g/L respectively,and it had the characteristics of low cost and high stability ability.The addition of VB_(1)(2.8 mg/L)and soybean oil(0.6 mL/L)could significantly enhance the carotenoid synthesis ability of JAASSRY strain,making it an ideal fermentation aid.

关 键 词：红酵母 类胡萝卜素 发酵助剂 

分 类 号：Q93-335[生物学—微生物学]