栀子油/米糠蛋白纳米乳液的高压微射流制备工艺优化及稳定性研究  

Preparation and Stability Study of Nanoemulsion of Gardenia Oil/Rice Bran Protein by High-Pressure Microfluidization Process

作　　者：郝慧敏[1] 靳学远 纵伟 HAO Huimin;JIN Xueyuan;ZONG Wei(Food Engineering Department,Hebi Vocational and Technical College,Hebi 458002,China;College of Clinical Medicine,Hainan Vocational University of Science andTechnology,Haikou 570100,China;Food and Bioengineering College,ZhengzhouUniversity of Light Industry,Zhengzhou 450000,China)

机构地区：[1]鹤壁职业技术学院食品工程学院,河南鹤壁458002 [2]海南科技职业大学临床医药学院,海南海口570100 [3]郑州轻工业大学食品与生物工程学院,河南郑州450000

出　　处：《食品科技》2024年第2期242-249,共8页Food Science and Technology

基　　金：海南省重点研发计划项目(ZDYF2021XDNY143);鹤壁职业技术学院科研课题(2023-KJZD-005)。

摘　　要：为制备栀子油纳米乳液,以栀子油和米糠蛋白为原料,采用高压微射流(High Pressure Microfluidizatio,HPM)技术制备栀子油/米糠蛋白纳米乳液。通过单因素试验研究HPM压力、米糠蛋白用量、栀子油用量和处理次数对纳米乳液平均粒径的影响,在此基础上,采用Box-Behnken试验设计,以纳米乳液平均粒径为响应值进行响应面优化,确定最优工艺参数并且对乳液的稳定性进行研究。结果表明:栀子油/米糠蛋白纳米乳液制备的最佳工艺参数为:HPM压力100 MPa、米糠蛋白添加量1.8%、栀子油添加量9.8%和处理次数2次,该条件下,纳米乳液平均粒径为279.9nm。纳米乳液在50~90℃、p H6~8条件下储藏28 d,Zeta电位绝对值均大于30 mV,具有较好的稳定性。研究表明HPM是制备栀子油/米糠蛋白纳米乳液适宜的方法。In order to prepare the nanoemulsion of gardenia oil,using gardenia oil and rice bran protein as raw materials,the high-pressure microfluidization(HPM) was employed for preparation nanoemulsion of gardenia oil/rice bran protein.The effects of pressure,rice bran protein dosage,gardenia oil dosage and treat times on average particle size of nanoemulsion were studied.Based on this,Box-Behnken design of experiments was adopted,and the average particle size of nanoemulsion was taken as the response value for response surface test to determine the optimal process parameters and study the stability of the emulsion.The results shown the optimum preparation conditions were as follows:pressure 100 MPa,rice bran protein dosage 1.8% and gardenia oil dosage 9.8% and treat 2 times.Under these conditions,the average particle size was 279.9 nm.The Zeta potential of nanoemulsion exceeded 30 mV at 50–90 ℃,pH6–8,and storage time of 28 days,respectively,and it has good stability.HPM was a suitable method for prepararing gardenia oil/rice bran protein nanoemulsion.

关 键 词：栀子油 米糠蛋白 纳米乳液 高压微射流 稳定性 

分 类 号：TS222.1[轻工技术与工程—粮食、油脂及植物蛋白工程]