Long-term exposure to aryl hydrocarbon receptor agonist neburon induces reproductive toxicity in male zebrafish(Danio rerio)  

作　　者：Fucong Zhang Chen Tang Yue Zhu Qian Wang Xin Huang Chunyan Yang Chengyong He Zhenghong Zuo 

机构地区：[1]Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems,School of Life Sciences,Xiamen University,Xiamen 361102,China

出　　处：《Journal of Environmental Sciences》2024年第8期193-203,共11页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.42177411)。

摘　　要：Neburon is a phenylurea herbicide that is widely used worldwide,but its toxicity is poorly studied.In our previous study,we found that neburon has strong aryl hydrocarbon receptor(AhR)agonist activity,but whether it causes reproductive toxicity is not clear.In the present study,zebrafish were conducted as a model organism to evaluate whether environmental concentrations of neburon(0.1,1 and 10μg/L)induce reproductive disorder in males.After exposure to neburon for 150 days from embryo to adult,that the average spawning egg number in high concentration group was 106.40,which was significantly lower than 193.00in control group.This result was mainly due to the abnormal male reproductive behavior caused by abnormal transcription of genes associated with reproductive behavior in the brain,such as secretogranin-2a.The proportions of spermatozoa in the medium and high concentration groups were 82.40%and 83.84%,respectively,which were significantly lower than 89.45%in control group.This result was mainly caused by hormonal disturbances and an increased proportion of apoptotic cells.The hormonal disruption was due to the significant changes in the transcription levels of key genes in the hypothalamus-pituitary-gonadal axis following neburon treatment.Neburon treatment also significantly activated the AhR signaling pathway,causing oxidative stress damage and eventually leading to a significant increase in apoptosis in the exposed group.Together,these data filled the currently more vacant profile of neburon toxicity and might provide information to assess the ecotoxicity of neburon on male reproduction at environmentally relevant concentrations.

关 键 词：ZEBRAFISH Neburon Male reproductive toxicity HPG axis AhR agonist 

分 类 号：X171.5[环境科学与工程—环境科学]