EPA/DHA型磷脂酰胆碱对MPP+诱导的SH-SY5Y细胞损伤的保护作用  

Protective Effect of EPA/DHA-rich Phosphatidylcholine on SH-SY5Y Cells Injured by MPP+

作　　者：葛丽君 卢蔚波 简士凯 郑慧敏 薛静 Ge Lijun;Lu Weibo;Jian Shikai;Zheng Huimin;Xue Jing(Institute of Seafood,Zhejiang Gongshang University,Hangzhou 310012;Collaborative Innovation Center of Seafood Deep Processing,Zhejiang Gongshang University,Hangzhou 310012)

机构地区：[1]浙江工商大学海洋食品研究院,杭州310012 [2]浙江工商大学海洋食品精深加工关键技术省部共建协同创新中心,杭州310012

出　　处：《中国食品学报》2024年第5期138-149,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC0311204)。

摘　　要：为探究EPA/DHA型磷脂酰胆碱(PCEPA/DHA)抗帕金森病(PD)的活性及其机制,采用MPP+诱导SH-SY5Y细胞构建PD体外模型,分析PCEPA/DHA干预对MPP+诱导的SH-SY5Y细胞活力、细胞形态及活性氧(ROS)水平等的影响,并结合转录组学技术筛选关键基因及通路。结果表明:1)PD造模的最佳条件为600μmol/L MPP+处理SH-SY5Y细胞24 h,PD模型组细胞相对活力下降(53.71±3.21)%;2)PCEPA/DHA可剂量依赖性增强MPP+诱导的SH-SY5Y细胞活力,改善细胞形态和降低细胞氧化应激水平;3)转录组学结果表明:PCEPA/DHA对MPP+诱导SH-SY5Y细胞的保护作用与改善线粒体功能障碍,改善多巴胺和突触核蛋白相关功能以及抑制细胞凋亡等密切相关。To investigate the anti-Parkinson's disease(PD)activity of EPA/DHA-rich phosphatidylcholine(PCEPA/DHA)and related mechanisms,MPP+-induced SH-SY5Y cells were used to construct a Parkinson's disease cell model,and the effects of EPA/DHA-phosphatidylcholine(PCEPA/DHA)on the viability,cell morphology and ROS levels of MPP+-induced SH-SY5Y cells were analyzed,and the mechanism was explored by transcriptomic techniques.The results showed that the optimal condition for PD modeling was 600μmol/L MPP+treatment of SH-SY5Y cells for 24 h.The relative cell viability of the PD model decreased by(53.71±3.21)%.PCEPA/DHA dose-dependently enhanced MPP+-induced SH-SY5Y cell viability,improved cell morphology and reduced cellular oxidative stress levels.Transcriptomic results showed that the protective effects of PCEPA/DHA on MPP+-induced SH-SY5Y cells were closely associated with improved mitochondrial dysfunction,improved dopamine and synuclein alpha-related functions,and inhibition of apoptosis.

关 键 词：EPA/DHA型磷脂酰胆碱 帕金森病 氧化应激 转录组学 

分 类 号：R742.5[医药卫生—神经病学与精神病学]