中华穿山甲尿液化学成分分析  

Analysis of the chemical components in the urine of the Chinese pangolin(Manis pentadactyla)

作　　者：吴文斌 李珺 王凯 安富宇 许学林 廖书佳 佟富春 华彦 WU Wenbin;LI Jun;WANG Kai;AN Fuyu;XU Xuelin;LIAO Shujia;TONG Fuchun;HUA Yan(Guangdong Academy of Forestry,Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China;Guangdong Wildlife Monitoring and Rescue Center,Guangzhou 510520,China)

机构地区：[1]广东省林业科学研究院,广东省森林培育与保护利用重点实验室,广州510520 [2]华南农业大学林学与风景园林学院,广州510642 [3]广东省野生动物监测救护中心,广州510520

出　　处：《兽类学报》2024年第3期385-393,共9页Acta Theriologica Sinica

基　　金：国家重点研发计划项目(2022YFF1301500)。

摘　　要：化学通讯在哺乳动物的诸多生命活动中起到重要作用,包括个体识别、社会交流、社会等级划分、交配繁殖等(侯东敏等,2020)。针对夜行性哺乳动物,化学通讯对其社会行为的调节作用尤为明显(Macdonald,1985)。尿液是动物机体新陈代谢的产物,也是哺乳动物化学通讯过程中重要的信号来源之一。在种内、种间信息交流的过程中,动物通过尿液能够传递个体、性别、年龄等讯息,以达到性别识别、性选择、告警、领域标记等目的,此过程对物种的生存、繁衍至关重要(Zhang et al.,2008;Zhang et al.,2019;鲜义坤等,2019;侯东敏等,2020)。Chemical communication is extremely important for many life activities of mammals,and urine is one of the pivotal signal sources in this process.Although pangolins use scent as their primary social medium,the study in the field of chemical communication for those animals is still unavailable.As a paradigmatic nocturnal mammal,urine could play a key role in the chemical communication for the Chinese pangolin(Manis pentadactyla).In this study,we analyzed the volatile components in the urine of Chinese pangolins(♀,n=3;♂,n=3)based on Headspace-Soild Phase Microextraction(HS-SPME)and Gas Chromatography-Mass Spectrometry(GC-MS).We found that 44 chemical compounds were detected from urine,including 17 ketones,nine aldehydes,five alkanes,four aromatic hydrocarbons,three esters,two olefins,two alcohols,one thioether,and one peroxide.32 and 38 chemical compounds of urine were found in females and males,respectively.There was sex dimorphism in the composition and relative content of volatile components of urine according to the results of PCA analysis.In males,di-tert-butyl peroxide and octane were unique,and acetone,2-pentanone,butyl acetate,and ethyl acetate had higher relative contents than those in females.Females had no specific components,but they had higher relative contents of hexane and methylcyclopentane than males.11 volatile components,including 3-penten-2-one,3-methylcyclopentanone,cyclohexanone,2-octanone,pentanal,decanal,3,3-dimethylhexane,ethylbenzene,4-methyl-1-hexene,5-ethyl-1,3-cyclohexadiene,and 6-methyl-hept-2-en-4-ol,were unique at individual level.Here,we characterized the volatile chemical components of urine in the Chinese pangolins,and demonstrated the differences of urine odor at gender and individual levels,and exhibited potential chemical communication functions of urine.

关 键 词：中华穿山甲 尿液 挥发性化合物 化学通讯 顶空固相微萃取 

分 类 号：Q491[生物学—生理学]