农贸市场食品来源大肠埃希菌的耐药性及其ESBL耐药基因的研究  

Study on the Resistance of Escherichia coli from Food Sources in Agricultural Markets and Its ESBL Resistance Genes

作　　者：郑月[1] 蔡鹏[1] 李艳 张云霞[1] 吴智云 王学龙[1] ZHENG Yue;CAI Peng;LI Yan;ZHANG Yunxia;WU Zhiyun;WANG Xuelong(Baoshan College of Traditional Chinese Medicine,Baoshan 678000,China)

机构地区：[1]保山中医药高等专科学校,云南保山678000

出　　处：《食品安全导刊》2024年第13期95-99,共5页China Food Safety Magazine

基　　金：云南省教育厅科学研究基金项目“食源标本分离多重耐药菌及其耐药基因的研究”(2021J1137)。

摘　　要：目的:调查云南省保山市农贸市场售卖畜禽肉蛋和蔬菜超广谱β-内酰胺酶(Extended-Spectrumβ-Lactamase,ESBL)大肠埃希菌污染情况,研究样品分离获得的大肠埃希菌ESBL耐药基因类型。方法:收集农贸市场食品样本,分离鉴定样品中的大肠埃希菌并进行药敏试验,扩增大肠埃希菌的ESBL基因,对扩增产物进行测序和同源性比对分析。结果:245份食品样本中分离获得大肠埃希菌75株,其中产ESBL大肠埃希菌25株。对ESBL耐药基因测序结果进行系统发育分析,发现耐药基因为CTX-M型,CTX-M-15序列有9株,占比为36%;CTX-M-14有16株,占比为64%。结论:肉类和蔬菜携带ESBL大肠埃希菌,多重耐药菌及其耐药基因可能通过食品在社区传播。Objective:To investigate the contamination of ESBL in Escherichia coli sold in livestock and poultry meat,eggs and vegetables in Baoshan city,Yunnan province,and to study the types of extended-spectrumβ-lactamase(ESBL)resistance genes of Escherichia coli isolated from the samples.Method:Food samples were collected in Baoshan farmers’market,and Escherichia coli was isolated and cultured for identification and drug susceptibility test,and ESBL gene was amplified by PCR,sequenced and analyzed for homology comparison.Result:A total of 75 strains of Escherichia coli were isolated from 245 food specimens,including 25 strains of ESBL E.coli.Phylogenetic analysis of the sequencing results of ESBL resistance genes showed that the drug resistance genes were CTX-M type,and there were 9 strains in the CTX-M-15 group,accounting for 36%.There were 16 strains in the CTX-M-14 group,and the detection rate was 64%.Conclusion:Meat and vegetables carry ESBL Escherichia coli,and multidrugresistant bacteria and their resistance genes may be transmitted in the community through food.

关 键 词：食品 大肠埃希菌 多重耐药菌 超广谱Β-内酰胺酶 耐药基因 

分 类 号：R155.5[医药卫生—营养与食品卫生学]