液相色谱-串联质谱法测定禽蛋及蛋制品中的苏丹红  

Determination of Sudan Dyes in Eggs and Egg Products by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：罗峰[1] 余健 黄亚梅 杨阳 黄立 LUO Feng;YU Jian;HUANG Yamei;YANG Yang;HUANG Li(Xishuangbanna Dai Autonomous Prefecture Agricultural Product Quality and Safety Testing Center,Xishuangbanna 666100,China;Xishuangbanna Dai Autonomous Prefecture Tea Industry Development Service Center,Xishuangbanna 666100,China;Kunming Food and Drug Inspection Institute,Kunming 650000,China)

机构地区：[1]西双版纳傣族自治州农产品质量安全检测中心,云南西双版纳666100 [2]西双版纳傣族自治州茶叶产业发展服务中心,云南西双版纳666100 [3]昆明市食品药品检验所,云南昆明650000

出　　处：《食品安全导刊》2024年第5期98-104,共7页China Food Safety Magazine

摘　　要：目的:建立液相色谱-串联质谱法测定禽蛋及蛋制品中的苏丹红残留量的分析方法。方法:乙腈提取,Captiva EMR-Lipid萃取柱净化;采用Waters ACQUITYUPLCBEHC_(18)(100 mm×2.1 mm,1.7μm)色谱柱,0.2%的甲酸水+乙腈为流动相梯度洗脱,流速为0.30 mL·min^(-1);电喷雾离子源,正离子扫描,多反应监测;开展方法学验证,评估了方法的线性范围、检出限、定量限、准确性、精密度和稳定性。结果:4种苏丹红残留液相色谱串联质谱法加标回收率在62.6%~110.0%,精密度相对标准偏差在1.20%~6.41%,检出限为5μg·kg^(-1),相关线性系数在0.997以上。结论:本方法通过乙腈提取萃取和Captiva EMR-Lipid萃取柱净化,有效去除干扰物质,并保证苏丹红的定量准确性,然后利用液相色谱-串联质谱仪进行分析,提高了检测灵敏度,该方法能够准确、可靠地测定禽蛋及蛋制品中苏丹红的残留量。该方法的建立为监督和控制禽蛋质量安全提供了一个重要的分析手段。Objective:To establish an analytical method for the determination of Sudan Red residues in poultry eggs and egg products by liquid chromatography tandem mass spectrometry.Method:Acetonitrile extraction,purification with Captiva EMR Lipid extraction column;waters ACQUITY UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)chromatographic column was used,with 0.2%formic acid water and acetonitrile as the mobile phase for gradient elution,with a flow rate of 0.30 mL·min^(-1);electro spray ion source,positive ion scanning,multi reaction monitoring;methodological validation was conducted to evaluate the linear range,detection limit,quantification limit,accuracy,precision,and stability of the method.Result:The recovery rates of the four Sudan Red residues by liquid chromatography tandem mass spectrometry were 62.6%~110.0%,with precision relative standard deviation ranging from 1.20%to 6.41%and a detection limit of 5μg·kg^(-1),with a correlation linear coefficient above 0.997.Conclusion:This method effectively removes interfering substances and ensures the quantitative accuracy of Sudan Red through acetonitrile extraction and purification with Captiva EMR Lipid extraction column.Then,liquid chromatography tandem mass spectrometry is used for analysis to improve detection sensitivity.This method can accurately and reliably determine the residual amount of Sudan red in poultry eggs and egg products.The establishment of this method provides an important analytical tool for monitoring and controlling the quality and safety of poultry eggs.

关 键 词：鸡蛋 鸭蛋 咸鸭蛋 苏丹红 液相色谱-串联质谱法 

分 类 号：TS253.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]