Investigating Müller glia reprogramming in mice: a retrospective of the last decade, and a look to the future  

作　　者：Zhiyuan Yin Jiahui Kang Xuan Cheng Hui Gao Shujia Huo Haiwei Xu 

机构地区：[1]Key Lab of Visual Damage and Regeneration&Restoration of Chongqing,Southwest Eye Hospital,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,China

出　　处：《Neural Regeneration Research》2025年第4期946-959,共14页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.31930068;National Key Research and Development Program of China,Nos.2018YFA0107302 and 2021YFA1101203(all to HX).

摘　　要：Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased states.In lower vertebrates like zebrafish,these cells assume responsibility for spontaneous retinal regeneration,wherein endogenous Müller glia undergo proliferation,transform into Müller glia-derived progenitor cells,and subsequently regenerate the entire retina with restored functionality.Conversely,Müller glia in the mouse and human retina exhibit limited neural reprogramming.Müller glia reprogramming is thus a promising strategy for treating neurodegenerative ocular disorders.Müller glia reprogramming in mice has been accomplished with remarkable success,through various technologies.Advancements in molecular,genetic,epigenetic,morphological,and physiological evaluations have made it easier to document and investigate the Müller glia programming process in mice.Nevertheless,there remain issues that hinder improving reprogramming efficiency and maturity.Thus,understanding the reprogramming mechanism is crucial toward exploring factors that will improve Müller glia reprogramming efficiency,and for developing novel Müller glia reprogramming strategies.This review describes recent progress in relatively successful Müller glia reprogramming strategies.It also provides a basis for developing new Müller glia reprogramming strategies in mice,including epigenetic remodeling,metabolic modulation,immune regulation,chemical small-molecules regulation,extracellular matrix remodeling,and cell-cell fusion,to achieve Müller glia reprogramming in mice.

关 键 词：cell fusion chemical small-molecules EPIGENETIC extracellular matrix immune metabolic MICE Müller glia neurodegenerative diseases REPROGRAMMING retina regeneration 

分 类 号：R338[医药卫生—人体生理学]