大豆7S球蛋白-大枣多糖复合物的制备、表征及抗原性  

Preparation,characterization and antigenicity of soybean 7S globulin-jujube polysaccharide complexes

作　　者：张晨婧 张西瑞 郭子瑶 韩丹丹[2] 窦海洋 ZHANG Chenjing;ZHANG Xirui;GUO Ziyao;HAN Dandan;DOU Haiyang(Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province,School of Basic Medical Sciences,Hebei University,Baoding 071000,China;College of Public Health,Hebei University,Baoding 071000,China)

机构地区：[1]河北大学基础医学院,河北省炎性自身免疫性疾病发病机制及防治重点实验室,河北保定071000 [2]河北大学公共卫生学院,河北保定071000

出　　处：《河北大学学报（自然科学版）》2024年第3期254-260,共7页Journal of Hebei University(Natural Science Edition)

基　　金：河北省重点研发项目(22372505D);河北省大学生创新创业训练计划项目(S202310075055);河北大学实验室开放项目(sy202245)。

摘　　要：为了降低大豆的抗原性,通过糖基化反应制备了大豆7S球蛋白-大枣多糖(7S-JP)复合物.采用非对称场流分离技术在线联用紫外可见光检测器、多角度光散射检测器和示差折光检测器(AF4-UVMALS-dRI)研究糖基化反应温度、时间和物料比对7S-JP复合物结构的影响,对7S-JP复合物的回转半径、摩尔质量和构象进行表征;采用间接竞争酶联免疫吸附法探究7S-JP复合物的抗原性.AF4-UV-MALS-dRI结果表明:在反应温度为70℃、反应时间为20 min时,7S与JP糖基化反应效果最佳;当7S与JP的质量比为1∶3时,形成的7S-JP复合物结构紧密,有利于破坏其空间抗原表位,抗原抑制率为(69.1±0.99)%.In order to reduce the antigenicity of soybean,the soybean 7S globulin-Jujube polysaccharide(7S-JP)complexes were prepared by glycosylation reaction.The effects of reaction temperature,reaction time,and material ratio of 7S to JP on the structure of 7S-JP complexes were studied by asymmetrical flow field-flow fractionation coupled with online ultraviolet-visible detector,multi-angle light scattering detector,and differential refractive index detector(AF4-UV-MALS-dRI).Meanwhile,the radius of gyration,molar mass,and conformation of 7S-JP complexes were characterized by AF4-UV-MALS-dRI.The antigenicity of 7S-JP complexes was studied by indirect enzyme-linked immunosorbent assay.The results showed that:The 7S reacted with JP sufficiently at 70℃in 20 min;The structure of 7S-JP complexes obtained with a material ratio of 1∶3 was more compact,which is beneficial to destroy its spatial epitope,and the antigen inhibition rate of 7S-JP(1∶3)achieved to be(69.1±0.99)%.

关 键 词：大豆7S球蛋白 大枣多糖 非对称场流分离 

分 类 号：O657[理学—分析化学]