Utilization of hydralazine as a reactive matrix for enhanced detection and on-MALDI-target derivatization of saccharides  

作　　者：Dan Ouyang Huan Huang Yanting He Jiajing Chen Jiali Lin Zhuling Chen Zongwei Cai Zian Lin 

机构地区：[1]Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology,Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety,College of Chemistry,Fuzhou University,Fuzhou 350108,China [2]School of Pharmacy,Bengbu Medical University,Bengbu 233000,China [3]Partner State Key Laboratory of Environmental and Biological Analysis,Department of Chemistry,Hong Kong Baptist University,Hong Kong,China

出　　处：《Chinese Chemical Letters》2024年第5期386-391,共6页中国化学快报（英文版）

基　　金：supported by a grant from the National Natural Science Foundation of China(Nos.22274021,21974021 and 22036001);the Natural Science Foundation of Fujian Province(No.2022J01535);the Major Project of Science and Technology of Fujian Province(No.2020HZ06006)。

摘　　要：Saccharides are a sort of ubiquitous and vital molecules within the whole life.However,the application of saccharides analysis with matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)is restricted by their low ionization efficiency and the instability of the sialic acid fraction.Derivatization strategy based on nonreductive amination provides a good solution,however,this is often time consuming and may result in sample loss due to removal of excessive derivatization reagents.Herein,hydralazine(HZN)was utilized as a reactive matrix for labeling reducing saccharides directly on MALDI target which eliminated tedious sample preparation and avoided sample loss.After optimization,effective and reproducible on-MALDI-target derivatization of neutral and acidic saccharides was achieved in both positive and negative modes.Compared with 2,5-dihydroxybenzoic acid(DHB)and 9-aminoacridine(9-AA),HZN improved the detection sensitivity of reducing saccharides and provided more abundant fragment ions in MS/MS analysis.Moreover,26 kinds of neutral glycans and 5 kinds of sialic glycans were identified from ovalbumin(OVA)and bovine fetuin,respectively.Combined with the statistical models,this strategy could be used to distinguish and predict samples of 6 brands of beer,and discriminate 2 kinds of beer fermentation modes.In addition,HZN was applied for quantitative analysis of glucose in urine samples,and the obtained urine glucose concentrations of diabetic patients were consistent with the clinical test results,showing the potential of qualitative and quantitative analysis of reducing saccharides in complex samples.

关 键 词：HYDRALAZINE Reactive matrix On-MALDI-target derivatization Saccharides 

分 类 号：TS262.5[轻工技术与工程—发酵工程] O652[轻工技术与工程—食品科学与工程]