Aspirin suppresses hepatocellular carcinoma progression by inhibiting platelet activity  

作　　者：Li-Jun Zhao Zhi-Yin Wang Wei-Ting Liu Li-Li Yu Hao-Nan Qi Jie Ren Chen-Guang Zhang 

机构地区：[1]Hematology Laboratory,The First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,Henan Province,China [2]Xinxiang Key Laboratory of Tumor Microenvironment and Immunotherapy,School of Medical Technology,Xinxiang Medical University,Xinxiang 453003,Henan Province,China [3]School of Public Health,Xinxiang Medical University,Xinxiang 453003,Henan Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2024年第6期2742-2756,共15页世界胃肠肿瘤学杂志（英文版）（电子版）

摘　　要：BACKGROUND Hepatocellular carcinoma(HCC)is the most common malignant liver disease in the world.Platelets(PLTs)are known to play a key role in the maintenance of liver homeostasis and the pathophysiological processes of a variety of liver diseases.Aspirin is the most classic antiplatelet agent.However,the molecular mechanism of platelet action and whether aspirin can affect HCC progression by inhibiting platelet activity need further study.AIM To explore the impact of the antiplatelet effect of aspirin on the development of HCC.METHODS Platelet-rich plasma,platelet plasma,pure platelet,and platelet lysate were prepared,and a coculture model of PLTs and HCC cells was established.CCK-8 analysis,apoptosis analysis,Transwell analysis,and real-time polymerase chain reaction(RT-PCR)were used to analyze the effects of PLTs on the growth,metastasis,and inflammatory microenvironment of HCC.RT-PCR and Western blot were used to detect the effects of platelet activation on tumor-related signaling pathways.Aspirin was used to block the activation and aggregation of PLTs both in vitro and in vivo,and the effect of PLTs on the progression of HCC RESULTS PLTs significantly promoted the growth,invasion,epithelial-mesenchymal transition,and formation of an inflammatory microenvironment in HCC cells.Activated PLTs promoted HCC progression by activating the mitogenactivated protein kinase/protein kinase B/signal transducer and activator of transcription three(MAPK/AKT/STAT3)signaling axis.Additionally,aspirin inhibited HCC progression in vitro and in vivo by inhibiting platelet activation.CONCLUSION PLTs play an important role in the pathogenesis of HCC,and aspirin can affect HCC progression by inhibiting platelet activity.These results suggest that antiplatelet therapy has promising application prospects in the treatment and combined treatment of HCC.

关 键 词：PLATELETS Antiplatelet therapy Hepatocellular carcinoma ASPIRIN ANTITUMOR 

分 类 号：R285[医药卫生—中药学]