Effects of interleukin-10 treated macrophages on bone marrow mesenchymal stem cells via signal transducer and activator of transcription 3 pathway  

作　　者：Meng-Hao Lyu Ce Bian Yi-Ping Dou Kang Gao Jun-Ji Xu Pan Ma 

机构地区：[1]Department of Periodontics,School of Stomatology,Capital Medical University,Beijing 100050,China [2]Department of Orthodontics,School of Stomatology,Capital Medical University,Beijing 100050,China [3]Department of Dental Implantology,School of Stomatology,Capital Medical University,Beijing 100050,China [4]Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction,School of Stomatology,Capital Medical University,Beijing 100050,China [5]Beijing Laboratory of Oral Health,Capital Medical University,Beijing 100050,China

出　　处：《World Journal of Stem Cells》2024年第5期560-574,共15页世界干细胞杂志（英文版）（电子版）

摘　　要：BACKGROUND Alveolar bone defects caused by inflammation are an urgent issue in oral implant surgery that must be solved.Regulating the various phenotypes of macrophages to enhance the inflammatory environment can significantly affect the progression of diseases and tissue engineering repair process.AIM To assess the influence of interleukin-10(IL-10)on the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)following their interaction with macrophages in an inflammatory environment.METHODS IL-10 modulates the differentiation of peritoneal macrophages in Wistar rats in an inflammatory environment.In this study,we investigated its impact on the proliferation,migration,and osteogenesis of BMSCs.The expression levels of signal transducer and activator of transcription 3(STAT3)and its activated form,phos-phorylated-STAT3,were examined in IL-10-stimulated macrophages.Subsequently,a specific STAT3 signaling inhibitor was used to impede STAT3 signal activation to further investigate the role of STAT3 signaling.RESULTS IL-10-stimulated macrophages underwent polarization to the M2 type through substitution,and these M2 macrophages actively facilitated the osteogenic differentiation of BMSCs.Mechanistically,STAT3 signaling plays a crucial role in the process by which IL-10 influences macrophages.Specifically,IL-10 stimulated the activation of the STAT3 signaling pathway and reduced the macrophage inflammatory response,as evidenced by its diminished impact on the osteogenic differentiation of BMSCs.CONCLUSION Stimulating macrophages with IL-10 proved effective in improving the inflammatory environment and promoting the osteogenic differentiation of BMSCs.The IL-10/STAT3 signaling pathway has emerged as a key regulator in the macrophage-mediated control of BMSCs’osteogenic differentiation.

关 键 词：MACROPHAGES INTERLEUKIN-10 Bone marrow mesenchymal stem cells Signal transducer and activator of transcription 3 Inflammatory response 

分 类 号：R318.08[医药卫生—生物医学工程]