Expression levels of K_(ATP)channel subunits and morphological changes in the mouse liver after exposure to radiation  

作　　者：Ming Zhou Tao-Sheng Li Hiroshi Abe Hideo Akashi Ryoji Suzuki Yoshio Bando 

机构地区：[1]Department of Anatomy,Akita University Graduate School of Medicine,Akita 010-8543,Japan [2]Department of Stem Cell Biology,Atomic Bomb Disease Institute,Nagasaki University,Nagasaki 852-8523,Japan [3]Sendai Old Age Refresh Station,A Long-term Care Health Facility,Sendai 981-1105,Japan

出　　处：《World Journal of Experimental Medicine》2024年第2期103-113,共11页世界实验医学杂志

基　　金：Supported by the Program of the Network-type Joint Usage/Research Center for Radiation Disaster Medical Science of Hiroshima University,Nagasaki University.

摘　　要：BACKGROUND ATP sensitive K+(K_(ATP))channels are ubiquitously distributed in various of cells and tissues,including the liver.They play a role in the pathogenesis of myocardial and liver ischemia.AIM To evaluate the radiation-induced changes in the expression of K_(ATP)channel subunits in the mouse liver to understand the potential role of K_(ATP)channels in radiation injury.METHODS Adult C57BL/6 mice were randomly exposed toγ-rays at 0 Gy(control,n=2),0.2 Gy(n=6),1 Gy(n=6),or 5 Gy(n=6).The livers were removed 3 and 24 h after radiation exposure.Hematoxylin and eosin staining was used for morphological observation;immunohistochemical staining was applied to determine the expression of K_(ATP)channel subunits in the liver tissue.RESULTS Compared with the control group,the livers exposed to 0.2 Gyγ-ray showed an initial increase in the expression of Kir6.1 at 3 h,followed by recovery at 24 h after exposure.Exposure to a high dose of 5.0 Gy resulted in decreased expression of Kir6.1 and increased expression of SUR2B at 24 h.However,the expression of Kir6.2,SUR1,or SUR2A had no remarkable changes at 3 and 24 h after exposure to any of these doses.CONCLUSION The expression levels of Kir6.1 and SUR2B in mouse liver changed differently in response to different radiation doses,suggesting a potential role for them in radiation-induced liver injury.

关 键 词：Radiation exposure ATP-sensitive K^(+)channel MOUSE LIVER 

分 类 号：R737.9[医药卫生—肿瘤]