piRNA hsa_piR_016187调控椎管内神经鞘瘤细胞增殖的实验研究  

Experimental study on piRNA hsa_piR_016187 regulating the proliferation of intraspinalschwannoma cells

作　　者：李庆伟[1,2] 宋建梅 李衍朋[1] 王传东 LI Qingwei;SONG Jianmei;LI Yanpeng;WANG Chuandong(Department of Spinal Surgery,Affiliated Hospital of Jining Medical University,Shandong Jining 272100,China;China Medical University,Liaoning Shenyang 110000,China;Department of Orthopedic Surgery,Xinhua Hospital Affiliated to Shanghai Jiao Tong University,Shanghai 310000,China)

机构地区：[1]济宁医学院附属医院脊柱外科,山东济宁272100 [2]中国医科大学,辽宁沈阳110000 [3]上海交通大学附属新华医院骨科,上海310000

出　　处：《现代肿瘤医学》2024年第11期1996-2000,共5页Journal of Modern Oncology

摘　　要：目的:探究PIWI相互作用RNA(PIWI-interacting RNA,piRNA)hsa_piR_016187对椎管内神经鞘瘤细胞增殖的调控作用及作用机制。方法:利用piRNA测序分析神经鞘瘤组织和对应的肿瘤旁正常组织中piRNA的表达情况,利用实时荧光定量PCR检测piRNA的表达,利用CCK-8检测和克隆增殖实验检测piRNA hsa_piR_016187对神经鞘瘤增殖的影响,利用双荧光素酶报告基因和Western blot检测piRNA hsa_piR_016187对胰岛素样生长因子2表达的调控作用。结果:piRNA hsa_piR_016187在神经鞘瘤组织中表达降低,piRNA hsa_piR_016187可以抑制神经鞘瘤细胞的增殖,双荧光素酶报告基因结果表明piRNA hsa_piR_016187可以和IGF23’-UTR区结合,Western blot检测显示过表达piRNA hsa_piR_016187抑制IGF2的表达,抑制piRNA hsa_piR_016187的表达促进IGF2的表达。结论:piRNA hsa_piR_016187通过调控IGF2的表达抑制神经鞘瘤细胞的增殖。Objective:To explore the effect and mechanism of the regulatory role of PIWI-interacting RNA(piRNA)hsa_piR_016187 on intraspinalschwannoma.Methods:piRNA sequencing was used to analyze the expression of piRNA in schwannoma tissues and normal tissues.Real-time fluorescent quantitative PCR was used to detect the expression of piRNA.CCK-8 detection and clone formation test were used to detect the effect of hsa_piR_016187 on the proliferation of schwannoma cells,and dual luciferase reporter gene and Western blot were used to detect the regulation of hsa_piR_016187 on IGF2 expression.Results:The expression of piRNA hsa_piR_016187 decreased in schwannoma tissue,and piRNA hsa_piR_016187 could inhibit the proliferation of schwannoma cells.The results of double luciferase reporter gene showed that piRNA hsa_piR_016187 could bind to IGF23'-UTR region.Western blot showed that overexpression of piRNA hsa_piR_016187 inhibited the expression of IGF2,while inhibition of piRNA hsa_piR_016187 expression promoted the expression of IGF2.Conclusion:piRNA hsa_piR_016187 inhibits the proliferation of schwannomas by regulating the expression of IGF2.

关 键 词：PIRNA hsa_piR_016187 椎管内神经鞘瘤 增殖 胰岛素样生长因子2 

分 类 号：R739.4[医药卫生—肿瘤]