超声辅助有机酸预处理玉米芯复合酶解工艺优化  

Optimization of ultrasonic-assisted compound enzymatic hydrolysis process of corncob pretreated with organic acid

作　　者：于德涵[1] 朱颖怡 黎莉[1] 武海玲 YU De-han;ZHU Ying-yi;LI Li;WU Hai-ing(School of Food and Pharmaceutical Engineering,Suihua University,Suihua 152061,Heilongjiang,China)

机构地区：[1]绥化学院食品与制药工程学院,黑龙江绥化152061

出　　处：《湖北农业科学》2024年第5期168-174,共7页Hubei Agricultural Sciences

基　　金：黑龙江省大学生创新创业训练计划项目(202110236017);黑龙江省教育厅基本科研业务费项目(YWK10236210231)。

摘　　要：为破坏玉米芯木质纤维素的紧密结构以利于酶解、发酵,先通过筛选试验确定最适有机酸及其浓度;再以复合酶解试验确定最佳玉米芯水解酶组成及配比;最后通过响应面试验优化最佳预处理工艺。结果表明,以10%的马来酸对玉米芯预处理后酶水解的最优工艺为料液比25∶1、超声功率310 W,在71℃条件下超声处理34 min,处理后残余物用纤维素酶、β-葡萄糖苷酶和木聚糖酶(1∶2∶1)在pH 4.8、50℃条件下复合酶解48 h。此条件下还原糖得率达34.49%,接近玉米芯中纤维素含量,证明了该预处理酶解工艺稳定、可靠,可为玉米芯发酵生产燃料乙醇提供前期技术支持。The purpose was to destroy the tight structure of corncob lignocellulose for enzymatic hydrolysis and fermentation.The opti-mum organic acid and its concentration were determined by screening experiment firstly.Then the optimal composition and ratio of hy-drolases for corncob were determined by compound enzymatic hydrolysis experiment.Finally,the optimal pretreatment process was op-timized by response surface experiment.The results showed that the optimum process of enzymatic hydrolysis of corncob pretreated with 10%maleic acid was as follows:the liquid-solid ratio of 25∶1,ultrasonic power of 310 W,and ultrasonic treatment time of 34 min at 71℃,and the residue was hydrolyzed with cellulase,β-glucosidase and xylanase(1∶2∶1)for 48 h at pH 4.8 and temperature 50℃.Under this condition,the yield of reducing sugar was 34.49%,which was close to the cellulose content in corncob.The method was proved to be stable and reliable,and could provide preliminary technical support for ethanol production from corncob by fermenta-tion.

关 键 词：超声辅助 有机酸预处理 玉米芯 酶解 工艺优化 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]