基于谱效关系筛选广西甜茶调节脂代谢活性质量标志物  

Screening Quality Markers of Regulating Lipid Metabolism Activity of Rubus suavissimus S.Lee Based on Spectral Effect Relationship

作　　者：樊兰兰 巫秋连 黄万方 姜蔓菁 潘东进 凌洁 FAN Lanlan;WU Qiulian;HUANG Wanfang;JIANG Manjing;PAN Dongjin;LING Jie(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Key Laboratory of Extraction Purification and Quality Analysis,Nanning 530200,China;Institute of Marine Drugs,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西高校中药提取纯化与质量分析重点实验室,广西南宁530200 [3]广西中医药大学海洋药物研究院,广西南宁530200

出　　处：《食品工业科技》2024年第13期17-29,共13页Science and Technology of Food Industry

基　　金：国家自然科学基金(81903918,31660095);广西自然科学基金(2020GXNSFAA159165);广西中医药大学一流学科项目(2019XK117);广西中医药大学桂派中医药传承创新团队(2022B005);广西壮瑶药重点实验开放课题(GXZYZZ2020-12);广西高等学校千名中青年骨干教师培育计划。

摘　　要：目的:基于谱效关系筛选广西甜茶调节脂代谢活性部位中的质量标志物。方法:采用95%乙醇提取广西甜茶,依次用石油醚、氯仿、乙酸乙酯、正丁醇进行萃取得到不同萃取部位,将不同萃取部位溶液作用于诱导分化后的3T3-L1前脂肪细胞,以油红O染色法和细胞甘油三酯释放量为指标,筛选活性部位。采用超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF-MS/MS)对活性部位进行成分分析和解析,基于灰色关联度法和偏最小二乘回归法分析活性部位共有峰与其调节脂代谢药效之间的谱效关系,筛选质量标志物。结果:乙酸乙酯及正丁醇萃取部位为调节脂代谢的活性部位,鞣花酸、矢车菊素-3-O-芸香糖苷、甜茶素和对映-贝壳杉-16-烯-19-羧酸-13-O-β-D-葡萄糖苷4个成分与调节脂代谢活性作用高度相关,可作为甜茶调节脂代谢活性的质量标志物。结论:基于谱效关系的广西甜茶质量标志物的研究,对阐明药效物质基础、筛选与药效相关的核心质量标志物、保证药材质量的可控性和合理应用有重要意义。Objective:The study was designed to test solvent extracts of Rubus suavissimus(RS)for compounds that regulate lipid metabolism and to measure their relative activity as quantitative markers of lipid metabolism-regulating activities based on the spectrum-effect relationship.Method:RS was extracted with 95%ethanol,the different extraction parts were successively extracted with petroleum ether,methylene chloride,ethyl acetate and n-butanol,respectively.The solution of different extraction parts was applied to the induced differentiated 3T3-L1 preadipocytes,and the oil red O staining and triglyceride release from the cells were determined to screen the best metabolism-regulating site.Analysis of different extraction sited by UPLC-Q-TOF-MS/MS technique to separate and identify the components.Based on specific assays,image intensity analysis,grey correlation and partial least squares regressions,the spectrum-effect relationship of the most abundant active components and their ability to regulate lipid metabolism were determined,and the quality markers were screened.Results:Ethyl acetate and n-butanol extraction site was the best metabolism-regulating site.Four components,including ellagic acid,centaurin-3-O-rutinoside,rubusoside and enantio-kauri-16-ene-19-carboxylic acid-13-O-β-D-glucoside were highly associated with metabolism-regulating effects.It could be used as quality markers for regulating lipid metabolism.Conclusion:Investigation of the quality markers of RS extracts based on the spectrum-effect relationship is of great importance for elucidating the pharmacodynamics,screening the core quality markers for therapeutic activity,and ensuring the safety and rational application of traditional medicines.

关 键 词：广西甜茶 3T3-L1细胞 脂代谢 谱效关系 质量标志物 

分 类 号：TS272[农业科学—茶叶生产加工]