甜玉米芯多糖纳米银的合成、表征及体外活性研究  

Study on Synthesis,Characterization and in Vitro Activity of Sweet Corncob Polysaccharide Nano-Silver

作　　者：王鑫[1] 周卓 遇世友 岳振歌 修伟业 马永强[1] WANG Xin;ZHOU Zhuo;YU Shiyou;YUE Zhenge;XIU Weiye;MA Yongqiang(School of Food Engineering,Harbin University of Commerce,Harbin 150028,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028

出　　处：《食品工业科技》2024年第13期58-66,共9页Science and Technology of Food Industry

基　　金：哈尔滨商业大学“青年创新人才”支持计划项目(2019CX31);哈尔滨商业大学产业化项目(22CZ16);2023年中国烹饪协会全国餐饮职业教育教学指导委员会一般课题(CYHZW-YB2023021)。

摘　　要：纳米银(AgNPs)在生物医药领域有广泛应用,但传统物理化学方法合成纳米银存在污染及产物毒性、耗时长等问题,因此探究纳米银的制备极其重要。以甜玉米芯多糖(SCP)为原料,采用绿色方法成功合成了甜玉米芯多糖纳米银(SCP-AgNPs)并对反应条件进行优化,使用透射电子显微镜(TEM)、X射线衍射仪、X光电子能谱仪、傅里叶变换红外光谱对生成的纳米银进行表征,并对其体外抗氧化及抗糖尿病能力进行探究。结果表明:SCP-AgNPs的最佳反应条件为:溶液中硝酸银浓度为0.75×10^(-3)mol/L,pH为10.0,反应80 min,所制备的SCP-AgNPs呈球状,平均粒径为13.5 nm。结构表现为面心立方晶体结构,确定其中Ag为Ag0状态。合成的SCP-AgNPs有较强的体外清除自由基作用。同时,SCP-AgNPs对α-葡萄糖苷酶和α-淀粉酶这两种糖尿病标记酶的活性具有良好的抑制作用。SCP-AgNPs对糖尿病关键酶具有显著的抑制效果,具备潜在抗糖尿病活性,是一种适合纳米生物医学应用的纳米药物,未来将广泛应用于食品加工、医学等领域。Silver nanoparticles(AgNPs)are widely used in the field of biomedicine.However,the synthesis of AgNPs by traditional physical and chemical methods has problems such as pollution,product toxicity,and time-consuming.Therefore,it is extremely important to explore the preparation of AgNPs.Silver nanoparticles(AgNPs)of sweet corncob polysaccharide(SCP-AgNPs)were successfully synthesized by green method using sweet corncob polysaccharide(SCP)as raw material and the reaction conditions were optimized.The generated AgNPs were characterized by transmission electron microscopy(TEM),X-ray diffraction,X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy,and their antioxidant and anti-diabetic abilities in vitro were investigated.The results showed that the optimum reaction conditions of SCP-AgNPs were as follows:The concentration of silver nitrate in the solution was 0.75×10^(-3) mol/L,the pH was 10.0,and the reaction time was 80 min.The prepared SCP-AgNPs were spherical with an average particle size of 13.5 nm.The structure showed a face-centered cubic crystal structure,and Ag was determined to be Ag0 state.The synthesized SCP-AgNPs had strong free radical scavenging effect in vitro.At the same time,SCP-AgNPs had a good inhibitory effect on the activity ofα-glucosidase andα-amylase.SCP-AgNPs has a significant inhibitory effect on key enzymes of diabetes and has potential anti-diabetic activity.It is a nano-drug suitable for nano-biomedical applications and will be widely used in food processing,medicine and other fields in the future.

关 键 词：甜玉米芯多糖 银纳米颗粒 表征 抗氧化 抗糖尿病 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]