鲍鱼多糖的大孔树脂纯化工艺及其免疫调节活性分析  

Purification Technology by Macroporous Resin and Immunomodulatory Activity of Abalone Polysaccharide

作　　者：曲航 吴奕 刘常武 杨海龙[2] 罗自生[3] 戴晨义 QU Hang;WU Yi;LIU Changwu;YANG Hailong;LUO Zisheng;DAI Chenyi(Zhejiang Baizhentang Food Co.,Ltd.,Wenzhou 325014,China;School of Life&Environmental Science,Wenzhou University,Wenzhou 325035,China;College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310030,China)

机构地区：[1]百珍堂生物科技(浙江)有限公司,浙江温州325014 [2]温州大学生命与环境科学学院,浙江温州325035 [3]浙江大学生物系统工程与食品科学学院,浙江杭州310030

出　　处：《食品工业科技》2024年第13期186-194,共9页Science and Technology of Food Industry

基　　金：浙江省温州市瓯海区重大科技攻关项目(G20210204);浙江省博士后科研项目择优资助项目(ZJ2022137)。

摘　　要：目的:为提高鲍鱼多糖品质,对鲍鱼多糖的大孔树脂纯化工艺进行优化,并对纯化前后多糖的免疫调节活性分析评价。方法:以多糖保留率、蛋白质脱除率和色素脱除率的加权综合得分为指标,考察6种不同型号的大孔树脂对鲍鱼多糖的纯化效果,利用单因素实验和响应面分析法优化大孔树脂的纯化工艺条件,并通过体外细胞实验对纯化前后鲍鱼多糖的免疫调节活性进行研究。结果:HPD100的加权综合得分最高,对鲍鱼多糖的纯化效果最佳;HPD100纯化多糖的最佳工艺参数为多糖与树脂质量比1:42、吸附时间3.10 h,吸附温度35℃,在此条件下多糖保留率为97.58%±1.58%,蛋白质脱除率为30.20%±0.66%,色素脱除率为45.78%±1.01%,加权综合平均得分61.83%;体外细胞实验结果表明,纯化后鲍鱼多糖的免疫调节活性显著增强,RAW264.7巨噬细胞的增殖活力提高,NO、TNF-α和IL-6细胞因子分泌水平大幅增加。结论:本研究获得鲍鱼多糖的最佳大孔树脂纯化工艺条件,纯化后鲍鱼多糖的生物活性显著增强,可为高活性鲍鱼多糖的制备提供理论依据。Objective:To improve the quality of abalone polysaccharide,the purification process by macroporous resins was optimized,and the immunomodulatory activities of polysaccharides before and after purification were evaluated.Methods:The purification effects of six kinds of macroporous resins on abalone polysaccharide were investigated with the comprehensive score of polysaccharide recovery rate,deproteinization rate and decolorization rate as the index.Singlefactor experiment and response surface analysis were employed to optimize the purification process parameters.The immunomodulatory activities of abalone polysaccharide before and after purification were studied by cell experiment in vitro.Results:HPD100 had the best purification effect on abalone polysaccharide with the highest comprehensive score.The optimal purification process parameters were as follows:Polysaccharide to resin mass ratio 1:42,adsorption time 3.10 h,adsorption temperature 35℃.Under these conditions,the polysaccharide recovery rate,deproteinization rate and decolorization rate were 97.58%±1.58%, 30.20%±0.66% and 45.78%±1.01%, respectively, and the resulting average comprehensive score was 61.83%. Furthermore, the in vitro cell experiment results showed that the immunomodulatory activity of abalone polysaccharide was markedly enhanced after purification by HPD100, with stronger viability of RAW264.7 macrophages and higher secretion of cytokines including NO, TNF-α and IL-6. Conclusions: An optimal purification process of abalone polysaccharide by macroporous resins was obtained, and the purified polysaccharide exhibited significantly improved biological activity. This research would provide a theoretical basis for preparation of highly bioactive abalone polysaccharide.

关 键 词：鲍鱼 多糖 纯化 大孔树脂 免疫调节活性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]