兰州百合多糖提取工艺优化及抗氧化活性研究  

Optimization of Extraction Process and Antioxidant Activity of Lilium davidii var.Unicolor Salisb Polysaccharide

作　　者：杨家珺 刘晓风[1] 古丽扎旦姆·达吾提 刘帆帆 马文锦 李军 YANG Jiajun;LIU Xiaofeng;Gulizadam Dawuti;LIU Fanfan;MA Wenjin;LI Jun(College of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China;Lanzhou Shibai Agricultural Biotechnology Co.,LTD,Lanzhou 730050,China)

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050 [2]兰州十百农业生物科技有限公司,甘肃兰州730050

出　　处：《食品科技》2024年第4期229-237,共9页Food Science and Technology

基　　金：2023年甘肃省科技专员专项(23CXGA0018);2023年甘肃省科技型中小企业技术创新基金项目(23CXGA0137);2023年兰州市知识产权计划项目(G202302)。

摘　　要：以兰州百合为原料,探究了生物酶法提取百合多糖得率的工艺条件,并对多糖的吸湿保湿性和抗氧化活性进行了研究。在单因素实验的基础上,选取加酶量、酶解时间、酶解温度进行3因素3水平Box-Benhnken实验,利用Design-Expert软件进行数据分析,得到最佳提取条件,对最佳提取条件下的多糖透析获得不同截留分子质量的百合多糖,并进行吸湿保湿和抗氧化活性研究。结果表明,百合多糖最佳提取条件为加酶量4%、酶解时间60 min、酶解温度55℃,在此条件下多糖得率为25.91%。通过吸湿保湿实验,筛选吸湿保湿性最佳的百合多糖截留分子质量片段3500~8000 u,在RH43%、68%、93%条件下放置72 h,3500~8000 u分子质量片段吸湿率最大,分别为62.67%、122.3%、119.2%,RH43%时3500~8000 u分子质量片段保湿率最大,为70.44%。体外抗氧化实验表明,截留分子质量3500~8000 u的百合多糖片段2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基与羟自由基清除率均高于未透析百合多糖。吸湿保湿实验对百合多糖截留分子质量片段的筛选,为百合多糖在化妆品方面的研究提供了初步基础。Using Lilium davidii var.Unicolor Salisb(Lanzhou lily)as raw materials,the process conditions for extracting polysaccharides from Lanzhou lily using enzymatic methods were explored,and the moisture absorption,moisturizing and antioxidant activities of polysaccharides were studied.On the basis of single factor experiments,Box Benhnken experiment was conducted with three factors and three levels,including enzyme dosage,enzyme hydrolysis time,and enzyme hydrolysis temperature.Data analysis was conducted using Design Expert software to obtain the optimal extraction conditions.The moisture absorption,moisturizing,and antioxidant activities of different intercepted fraction of lily polysaccharides obtained under the optimal extraction conditions by polysaccharide dialysis were studied.The results showed that the optimal extraction conditions for lily polysaccharides were enzyme addition of 4%,enzymatic hydrolysis time of 60 minutes,and enzymatic hydrolysis temperature of 55℃.Under these conditions,the polysaccharide yield was 25.91%.Through moisture absorption and moisturizing experiments,the best moisture absorption and moisturizing properties of lily polysaccharides were screened by intercepting molecular weight fragments of 3500-8000 u.After being placed under RH 43%,68%,and 93%conditions for 72 hours,the moisture absorption rates of 3500-8000 u molecular weight fragments were the highest,accounting for 62.67%,122.3%,and 119.2%,respectively.At RH 43%,the moisture retention rates of 3500-8000 u molecular weight fragments were the highest,accounting for 70.44%.In vitro antioxidant experiments showed that 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)radical cation and hydrogen peroxide scavenging activities of 3500-8000 u fragments were higher than those of lily polysaccharide.The screening of molecular weight fragments intercepted by lily polysaccharides through moisture absorption and moisturizing tests provides a preliminary basis for the research of lily polysaccharides in cosmetics.

关 键 词：兰州百合 多糖 生物酶法 吸湿保湿性 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]