基于纯培养法结合高通量测序技术解析胀袋辐照凤爪中的微生物多样性  

Analysis of microbial diversity in swollen irradiated chicken feet based on pure culture method and high-throughput sequencing technology

作　　者：贾鑫 高鹏[2,3] 周瑞 徐攀 王丹 黄敏[2,3] 孟爱莲[2,3] 张雁君 吴舸洋 JIA Xin;GAO Peng;ZHOU Rui;XU Pan;WANG Dan;HUANG Min;MENG Ailian;ZHANG Yanjun;WU Geyang(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China;Sichuan Institute of Atomic Energy,Chengdu 610101,China;Irradiation preservation Key Laboratory of Sichuan Province,Chengdu 610101,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川省原子能研究院,四川成都610101 [3]辐照保藏四川省重点实验室,四川成都610101

出　　处：《食品与发酵工业》2024年第12期135-141,共7页Food and Fermentation Industries

基　　金：四川省重大科技专项(2019ZDZX0003);四川省自然科学基金项目(面上项目)(2023NSFSC0133);四川省科技创新基地(平台)基本科研业务(2023JDKY0009)。

摘　　要：为研究真空包装胀袋辐照凤爪中的微生物多样性,该研究采用纯培养法与高通量测序技术对其微生物多样性进行解析。纯培养法结果表明,13株菌经鉴定分为4株植物乳杆菌(Lactiplantibacillus plantarum)、3株短乳杆菌(Lactobacillus brevis)、1株寡养单胞菌(Stenotrophomonas maltophilia)、3株欺诈明串珠菌(Leuconostoc fallax)以及2株热带假丝酵母(Candida tropicalis)。高通量测序的结果表明,乳杆菌属(Lactobacillus)在胀袋辐照凤爪中的相对丰度最高(平均占比97.30%),是胀袋凤爪中的优势菌。此外,产气胀袋验证试验的结果表明L.brevis及L.fallax可引起产品胀袋。该研究结果可为掌握辐照凤爪胀袋产品中微生物污染及群落组成提供一定参考,并为后续产品微生物污染控制及产品品质提升提供一定理论依据。To investigate the microbial diversity of vacuum-packaged swollen irradiated chicken feet,the microbial diversity was analyzed in this study using the pure culture method and high-throughput sequencing technology.The pure culture approach revealed the identification of 13 strains,comprising 4 strains of Lactiplantibacillus plantarum,3 strains of Lactobacillus brevis,1 strain of Stenotrophomonas maltophilia,3 strains of Leuconostoc fallax,and 2 strains of Candida tropicalis.The results of high-throughput sequencing showed that Lactobacillus exhibited the highest relative abundance(averaging at 97.30%)and was the dominant bacterium in the swollen chicken feet.Additionally,gas production verification experiments revealed that L.brevis and L.fallax could cause bag swelling.The results of this study can provide a certain reference for mastering the microbial contamination and community composition of swollen irradiated chicken feet products,and provide a solid theoretical foundation for the subsequent control of microbial contamination and enhancement of product quality.

关 键 词：胀袋 辐照凤爪 纯培养法 高通量测序 微生物多样性 

分 类 号：TS251.55[轻工技术与工程—农产品加工及贮藏工程]