传统泡菜中高效降解核苷乳酸菌的筛选及其特性研究  

Isolation of efficient nucleoside-degrading lactic acid bacteria from traditional pickles and their probiotic properties

作　　者：李杰 张鹏霞[2] 周健[1] 贺敏[3] 彭景贤[3] 包艳[4] 宋丽新 LI Jie;ZHANG Pengxia;ZHOU Jian;HE Min;PENG Jingxian;BAO Yan;SONG Lixin(School of Public Health,Jiamusi University,Jiamusi 154000,China;School of Basic Medical Sciences,Jiamusi University,Jiamusi 154000,China;Baotou Disease Prevention Control Center,Baotou 014030,China;School of Public Health,Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014060,China)

机构地区：[1]佳木斯大学公共卫生学院,黑龙江佳木斯154000 [2]佳木斯大学基础医学院,黑龙江佳木斯154000 [3]包头市疾病预防控制中心,内蒙古包头014030 [4]内蒙古科技大学包头医学院公共卫生学院,内蒙古包头014060

出　　处：《食品与发酵工业》2024年第12期227-233,共7页Food and Fermentation Industries

基　　金：佳木斯大学青年创新人才培养支持计划项目(JMSUQP2022014);黑龙江省省属高等学校基本科研业务费科研项目(2022-KYYWF-0649);黑龙江省“双一流”学科协同创新成果项目(LJGXCG2023-089);内蒙古自治区卫生健康科技计划项目(202201527)。

摘　　要：高尿酸血症是一种因尿酸代谢障碍而引发的一种代谢性疾病。乳酸菌作为一类重要的益生菌可通过多种方式改善高尿酸血症。该研究旨在从泡菜中筛选能够高效降解核苷的乳酸菌并分析其特性。利用高效液相色谱法分析所筛选菌株的核苷降解能力,检测菌株对黄嘌呤氧化酶抑制作用、体外环境耐受性及抗生素敏感性。结果表明,短乳杆菌(Levilactobacillus brevis)SLlac-18对肌苷和鸟苷的降解率分别可达97.81%和97.89%;且对黄嘌呤氧化酶的活力具有一定抑制作用。此外,在pH 2.0的环境下,SLlac-18的存活率可达96%;在3 g/L胆盐环境下处理5 h条件下,SLlac-18的存活率可达96.33%;而在胃肠液环境中,SLlac-18同样表现较高的耐受性,存活率均在90%以上,且菌株对红霉素、氨苄西林等4种常见抗生素具有敏感性。短乳杆菌SLlac-18具有潜在降尿酸功能,且具有良好的益生特性,可以应用于高尿酸血症预防和辅助治疗中,具有一定应用价值。Hyperuricemia is a metabolic disease caused by uric acid metabolism disorder.Lactic acid bacteria as an important probiotic could ameliorate hyperuricemia in multiple ways.The study aimed to isolate lactic acid bacteria with capable to degrade nucleosides efficiently from pickles.The nucleoside degradation ability of the screened strains was analysed by using high performance liquid chromatography(HPLC),then the xanthine oxidase inhibition by strains,environmental tolerance in vitro and antibiotic susceptibility were also tested.Results showed that the degradation rate of inosine and guanosine by lactobacillus brevis SLlac-18 were 97.81%and 97.89%,respectively,and xanthine oxidase inhibition of strains were also existed.In addition,the survival rate of SLlac-18 was 96%at pH 2.0,96.33%at 3 g/L bile salt for 5 h,and 96.33%at gastrointestinal fluid for 5 h.SLlac-18 was also highly tolerant to the treatment of gastrointestinal fluids with a survival rate of more than 90%,and was susceptible to four common antibiotics such as erythromycin and ampicillin.lactobacillus brevis SLlac-18 has the potential function to degrade uric acid,which can be applied to the prevention and treatment of hyperuricemia,that has certain application value.

关 键 词：高尿酸血症 核苷 乳酸菌 泡菜 益生菌 

分 类 号：TS255.54[轻工技术与工程—农产品加工及贮藏工程] TS201.3[轻工技术与工程—食品科学与工程]