StGID1基因调控马铃薯植株高度、直径和叶绿素含量  

Regulation Effect of StGID1 Gene on Potato Plant Height,Diameter and Chlorophyll Content

作　　者：李华鹏[1] 彭小荷 梁晓[1] 唐铭霞[1] 王克秀[1] 胡建军[1] LI Huapeng;PENG Xiaohe;LIANG Xiao;TANG Mingxia;WANG Kexiu;HU Jianjun(Crop Research Institute of Sichuan Academy of Agriculture Sciences/Environment-friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province,Chengdu 610066)

机构地区：[1]四川省农业科学院作物研究所/粮油作物绿色种质创新与遗传改良四川省重点实验室,成都610066

出　　处：《中国农学通报》2024年第15期102-109,共8页Chinese Agricultural Science Bulletin

基　　金：四川省科技计划项目“马铃薯GID1基因的克隆与表达分析”(2019YJ0597);四川省薯类创新团队项目“特色优质马铃薯育种及育种技术研究”(sccxtd-2022-09)。

摘　　要：为了研究赤霉素不敏感矮化基因(gibberellin insensitive dwarf,GID1)在马铃薯生长中的作用,在马铃薯中克隆该基因并转化植株记录相关表达差异。以马铃薯‘川芋21’组培苗为材料,通过马铃薯基因库设计引物克隆到了一个马铃薯赤霉素不敏感矮化基因StGID1。通过转化‘鄂马铃薯3号’,得到过表达转基因植株和RNAi植株并记录表达差异。进化分析显示,StGID1蛋白氨基酸序列与番茄SlGID1b-1基因的氨基酸序列同源性最高。过量表达和干扰抑制表达的转基因植株对比显示,在组培苗中,干扰抑制表达的植株平均茎直径为过量表达植株的2倍。在成熟植株中,抑制表达植株株高仅为过表达植株株高的62.8%,显著低于空白对照和过表达植株。在赤霉素处理时,过表达转基因植株、干扰抑制表达植株及对照均表现出随赤霉素浓度升高而茎高增高,茎直径减小。本研究证明StGID1是GID1基因家族在马铃薯中的同源基因,为马铃薯赤霉素调控路径的一部分。In order to investigate the role of gibberellin insensitive dwarf gene(GID1)in potato growth,the gene was cloned and transformed to record the expression differences.StGID1 was cloned from‘Chuanyu 21’tissue culture seedlings by using the primers designed from PGSC(Potato Genomics Resource).By transformation into potato‘E Malingshu 3’,overexpressing transgenic plants and RNAi plants were obtained,and the differences in expression were recorded.Phylogenetic analysis showed that the amino acid sequence of StGID1 protein had the highest homology with SlGID1b-1 in tomato.The comparison of overexpression transgenic plants and suppressed expression transgenic plants showed that in the tissue culture seedlings,the average stem diameter of suppressed expression transgenic plants was twice that of overexpression plants.In the mature plants,the relative height of the suppressed expression plants was only 62.8%of that in the overexpressed plants,significantly lower than the blank control and overexpressed plants.When treated with gibberellin,it showed an increase in stem height and a decrease in stem diameter in overexpression plants,suppressed expression plants,and control,with increasing of gibberellin concentration.This study verified that StGID1 was a homologous gene of GID1 gene family in potato and was a part of the gibberellin regulation pathway in potato.

关 键 词：马铃薯 StGID1 系统进化分析 赤霉素调控路径 

分 类 号：S532[农业科学—作物学]