大豆乳清蛋白作为氮源和天然浮选剂用于培养及采收小球藻的工艺研究  

A study on the cultivation and harvest process of Chlorella by foam flotation:Whey soy protein as nitrogen source and bio-surfactant

作　　者：梁烽 黄迪 高双飞 王腾飞[1] 张川川 李沙 LIANG Feng;HUANG Di;GAO Shuangfei;WANG Tengfei;ZHANG Chuanchuan;LI Sha(Faculty of Biology Engineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250353,China;Gaoqing Inspection and Testing Center,Zibo 256399,China;Shandong Fuxin Biotechnology Co.,Ltd.,Zibo 256300,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生物工程学部,山东济南250353 [2]高青县检验检测中心,山东淄博256399 [3]山东富欣生物科技股份有限公司,山东淄博256300

出　　处：《齐鲁工业大学学报》2024年第3期56-63,共8页Journal of Qilu University of Technology

基　　金：山东省自然科学基金(ZR2021QB074);山东省大学生创新创业训练计划项目(S202310431137);齐鲁工业大学人才科研项目(2023RCKY225);山东省重点研发项目(2022CXGC010506)。

摘　　要：微藻作为第三代生物质原料,生产能力高、产油脂量高,可以用于制备生物柴油。为了有效的提高微藻生产及分离效率,泡沫浮选法被用于解决微藻采收及脱水困难的问题。蛋白质作为天然氮源及天然表面活性剂,在设计小球藻的连续培养-分离工艺中起到的关键桥梁的作用。从大豆生产企业排放的大豆乳清废水中提纯出大豆乳清蛋白加以利用,很好的降低了有机氮源的成本。以大豆乳清蛋白作为小球藻培养的有机氮源被证明有显著的促生长作用。同时大豆乳清蛋白作为捕获剂和起泡剂,在最佳补入质量浓度为0.163 g/L,pH 7.0条件下得到小球藻的回收率为96.4%,富集比为18.7。Microalgae,the third generation biomass raw material,has been used to produce biodiesel for its advantages of high production capacity and high lipids content.In order to increase the production and separation efficiency of microalgae,foam flotation can be applied in the harvest of microalgae.Protein as a natural nitrogen source and a bio-surfactant can play a role of a bridge in coupling the cultivation process with the separation process.The application of the recovered whey soy protein from the soy whey wastewater is cost-effective to the production of Chlorella.The addition of whey soy protein was beneficial to the growth of Chlorella in BBM.In foam flotation,a high enrichment ration 18.7 and a high recovery percentage 96.4%of Chlorella were obtained by adding the optimal whey soy protein concentration of 0.163 g/L at pH 7.0.

关 键 词：泡沫浮选 大豆乳清蛋白 小球藻 天然浮选剂 微藻采收 

分 类 号：TQ028.8[化学工程] Q819[生物学—生物工程]