基于秀丽线虫构建生态环境健康课程链式实验教学体系  

Establishment of Caenorhabditis elegans-based Experimental Teaching S ystem for Curriculum Chain of Ecological Environmental Health

作　　者：于振洋[1,2,3] Rafeeq Karam Mustafa Mustafa 尹大强 Yu Zhenyang;Rafeeq Karam Mustafa Mustafa;Yin Daqiang(College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China;Shanghai Institute of Pollution Control and Ecological Security,Shanghai 200092,China;Jiaxing Tongji Institute for Environment,Jiaxing 314051,China)

机构地区：[1]同济大学环境科学与工程学院,上海200092 [2]上海污染控制与生态安全研究院,上海200092 [3]嘉兴同济环境研究院,嘉兴314051

出　　处：《生态毒理学报》2024年第3期124-130,共7页Asian Journal of Ecotoxicology

基　　金：上海市科学技术委员会自然科学基金(STCSM No.21ZR1468200);国家自然科学基金(No.21976138)。

摘　　要：我国生态环境健康领域不断发展,生态环境健康教学逐渐形成完善的教育体系与课程链,但目前尚缺乏与课堂理论教学相匹配的实验教学体系,对培养创新思维与实践能力兼备的专业人才提出了新的挑战。秀丽线虫,生物学背景雄厚,是生命科学、生态环境健康中广泛使用的模式生物。本文以秀丽线虫为模式动物,构建较为完整的实验内容和案例教学体系,涵盖培养基配制、微生物显微观测、个体生长发育、生殖能力、总蛋白含量、催化酶活性、基因表达水平等方面内容与检测方法,与微生物学、分子生物学、生物化学、环境毒理学、生态毒理学等相关理论体系匹配,锻炼学生实践动手与实验设计技能,为学生后续围绕科学问题开拓创造性思维、提升科研能力奠定基础。The field of ecological environment health is developing continuously in our country,and it has already formed a well-developed education system and curriculum chain.However,it still lacks an experimental teaching system in laboratories to match the theoretical education in classrooms.Such a lack brings challenges in educating professionals integrated with innovative spirits and practical abilities.Caenorhabditis elegans has solid biological background information and is a model organism in the fields of life science and ecological environmental health.An experimental teaching system is established with C.elegans in the present study.It includes preparation of culture medium,microscopic observation,individual development,reproduction,total protein contents,enzyme activities and also gene expression levels.The overall contents strongly match the theoretical contents in microbiology,biochemistry,environmental toxicology and ecological toxicology.The experimental teaching system can improve the students’capacities in practical operation and experimental design,which will pave ways for their innovative spirits and practical abilities to solve scientific problems in the future.

关 键 词：生态环境健康 秀丽线虫 实验教学体系 课程链 

分 类 号：X171.5[环境科学与工程—环境科学]