Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation  

作　　者：Yiduo Liu Junjie Hu Bing Yan 

机构地区：[1]National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 101408,China

出　　处：《Biophysics Reports》2024年第2期61-66,共6页生物物理学报（英文版）

基　　金：supported by grants from the National Key R&D Program of China(2021YFA1300800);Project for Young Scientists in Basic Research(YSBR-075)of the Chinese Academy of Sciences。

摘　　要：The endoplasmic reticulum(ER)is an essential component of the endomembrane system in eukaryotes and plays a crucial role in protein and lipid synthesis,as well as the maintenance of calcium homeostasis.Morphologically,the ER is composed primarily of sheets and tubules.The tubular ER is composed of a network of tubular membrane structures,each with diameters ranging from 30 to 50nanometers.In recent years,there has been in-depth research on the molecular mechanisms of membrane shaping and membrane fusion of the tubular ER.However,there is still limited understanding of the specific physiological functions of the tubular ER.Here,we report a protocol that combines differential centrifugation and immunoprecipitation to specifically enrich microsomes originating from the tubular ER in yeast.The ER tubule-derived microsomes can be further used for proteomic and lipidomic studies or other biochemical analyses.

关 键 词：Endoplasmic reticulum MICROSOME Differential centrifugation IMMUNOPRECIPITATION 

分 类 号：Q244[生物学—细胞生物学]