云南高校图书馆联盟文献共享服务平台- IMMUNOPRECIPITATION

IMMUNOPRECIPITATION: 作品数：21被引量：17H指数：2; 导出分析报告; 相关领域：医药卫生生物学更多>>; 相关作者：王敬伟冷炫李杰胡洁杨细飞更多>>; 相关机构：深圳市疾病预防控制中心中山大学华南理工大学更多>>; 相关期刊：《World Journal of Medical Genetics》《Chemical Research in Chinese Universities》《Chinese Journal of Cancer Research》《热带病与寄生虫学》更多>>; 相关基金：国家自然科学基金广东省科技计划工业攻关项目国家高技术研究发展计划更多>>

Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation: 《Biophysics Reports》2024年第2期61-66,共6页Yiduo Liu Junjie Hu Bing Yan; supported by grants from the National Key R&D Program of China(2021YFA1300800);Project for Young Scientists in Basic Research(YSBR-075)of the Chinese Academy of Sciences。; The endoplasmic reticulum(ER)is an essential component of the endomembrane system in eukaryotes and plays a crucial role in protein and lipid synthesis,as well as the maintenance of calcium homeostasis.Morphologically...; 关键词：Endoplasmic reticulum MICROSOME Differential centrifugation IMMUNOPRECIPITATION

RNA binding protein BOULE forms aggregates in mammalian testis: 《The Journal of Biomedical Research》2022年第4期255-268,共14页Yujuan Su Xinghui Guo Min Zang Zhengyao Xie Tingting Zhao Eugene Yujun Xu; supported by the National Natural Science Foundation of China(No.3197060323);SKLRM grant(SKLRM-2019B2);the Jiangsu ShuangChuang Talent Program,as well as the Jiangsu graduate student innovation fellowship to Y.S.; Amyloids have traditionally been considered pathologic protein aggregates which contribute to neurodegeneration.New evidence however increasingly suggests that non-pathological amyloids are formed in animals during no...; 关键词：AMYLOID RNA-binding protein BOULE protein aggregation SDD-AGE enhanced UV cross-linking immunoprecipitation

Characterization of endogenous nucleic acids that bind to NgAgo in Natronobacterium gregoryi sp2 cells: 《BIOCELL》2022年第2期547-557,共11页LIXU JIANG LIN NING CHUNCHAO PU ZIXIN WANG BIFANG HE JIAN HUANG; The datasets generated during the current study are available in the Sequence Read Archive(SRA)repository under the accession number PRJNA720376(BioProject);GenBank under the accession number PKKI00000000.; As nucleic acid-guided endonucleases,some prokaryotic Argonautes have been used as programmable nucleases.Natronobacterium gregoryi Argonaute(NgAgo)has also been proposed for gene editing,but this remains very controv...; 关键词：NgAgo PIWI IMMUNOPRECIPITATION CHIP-SEQ RIP-seq Post-transcriptional regulation

The Effect of Elution Volume for Immunoprecipitation on m6A-Seq Analysis: 《Proceedings of Anticancer Research》2021年第4期1-5,共5页Yuhui Xu Lize Shen Guolin Li; Objective:To develop a cost-effective method to reduce the time consumption of elution in immunoprecipitation.Methods Two volumes(125μL for Group C and 100μL for Group T)of elution buffer were used to explore whethe...; 关键词：m^(6)A-Seq IMMUNOPRECIPITATION Elution buffer

Long non-coding RNA SAP30-2:1 is downregulated in congenital heart disease and regulates cell proliferation by targeting HAND2被引量：2: 《Frontiers of Medicine》2021年第1期91-100,共10页Jing Ma Shiyu Chen Lili Hao Wei Sheng Weicheng Chen Xiaojing Ma Bowen Zhang Duan Ma Guoying Huang; supported by grants from the National Key Research and Development Program of China(No.2016YFC1000500);the National Science Foundation for Young Scientists(No.81801501);the Postdo ctoral Science Foundation of China(No.2018M632026).; Congenital heart disease(CHD)is the most common birth defect worldwide.Long non-coding RNAs(lncRNAs)have been implicated in many diseases.However,their involvement in CHD is not well understood.This study aimed to inv...; 关键词：congenital heart disease Gene Expression Omnibus lncRNA SAP30-2:1 cell proliferation RNA immunoprecipitation HAND2

A Bayesian hierarchical model for analyzing methylated RNA immunoprecipitation sequencing data被引量：2: 《Frontiers of Electrical and Electronic Engineering in China》2018年第3期275-286,共12页Minzhe Zhangl Qiwei Lil Yang Xie; Background： The recently emerged technology of methylated RNA immunoprecipitation sequencing （MeRIP-seq） sheds light on the study of RNA epigenetics. This new bioinformatics question calls for effective and robust ...; 关键词：MeRIP-seq data RNA epigenomics Bayesian inference hidden Markov model zero-inflated negativebinomial

Development and characterization of monoclonal antibodies against human aryl hydrocarbon receptor: 《Journal of Environmental Sciences》2016年第1期165-174,共10页Wenjing Tian Xinhui Pei Heidi Qunhui Xie Sherry Li Xu Jijing Tian Qin Hu Haiming Xu Yangsheng Chen Hualing Fu Zhengyu Cao Bin Zhao; supported by the National Natural Science Foundation of China (Nos. 21277168, 21525730);the Strategic Priority Research Program of the Chinese Academy of Sciences (Nos. XDB14030401, XDB14030402); Aryl hydrocarbon receptor（Ah R）, a ligand-dependent nuclear receptor, is involved in a diverse spectrum of biological and toxicological effects. Due to the lack of three dimensional（3D）crystal or nuclear magnetic ...; 关键词：Aryl hydrocarbon receptor Monoclonal antibody Western blot Immunoprecipitation Indirect immunofluorescence assay

GAS2-Calpain2 axis contributes to the growth of leukemic cells被引量：2: 《Acta Biochimica et Biophysica Sinica》2015年第10期795-804,共10页LUi Sun Haixia Zhou Hong Liu Yue Ge Xiuyan Zhang Wenjuan Ma Depei Wu Yun Zhao; These authors are grateful to the generous gift of spermine-introduced pullulan as a robust carrier for siRNA delivery from Drs Jian Liu and Haiyan Xu （Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing, China）.This work was supported by the grants from the National Natural Science Foundation of China （Nos. 31371392 and 81400113）, the National Key Scientific Project of China （No. 2011CB933501）, the Priority Academic Program Development of Jiangsu Higher Education Institutions （PAPD）, Jiangsu Province＇s Key Medical Center （ZX201102）, and National Public Health Grand Research Foundation （No. 201202017）.; Growth arrest specific 2 （GAS2） modulates cell cycle, apoptosis, and Calpain activity. GAS2-Calpain2 axis is required for the growth of BCR-ABL＋ hematopoietic cells and chronic myeloid leukemia cells. However, the ...; 关键词：GAS2 Calpain2 RNAI GAS2DN IMMUNOPRECIPITATION

Comparison of next generation sequencing-based and methylated DNA immunoprecipitation-based approaches for fetal aneuploidy non-invasive prenatal testing: 《World Journal of Medical Genetics》2015年第2期23-27,共5页Georgia Christopoulou Elisavet A Papageorgiou Philippos C Patsalis Voula Velissariou; Over the past few years, many researchers have attempted to develop non-invasive prenatal testing methods in order to investigate the genetic status of the fetus. The aim is to avoid invasive procedures such as chorio...; 关键词：Next generation sequencing Differential METHYLATION Epigenetics Fetal ANEUPLOIDY METHYLATION dependent IMMUNOPRECIPITATION NON-INVASIVE prenatal testing

低氧诱导人视网膜微血管内皮细胞核中乙酰肝素酶对血管内皮生长因子基因转录调控研究被引量：2: 《中国实用眼科杂志》2013年第9期1195-1199,共5页王敬伟胡洁宋新冷炫田景毅吕林; 国家自然科学基金面上项目（81271009）;广东省科技计划社会发展项目（20118020800005）;; 目的通过观察低氧诱导人视网膜血管内皮细胞（HRECs）胞核中乙酰肝素酶（HPA）和RNA聚合酶II（Pol Ⅱ）的表达分布、两者相互结合及其与血管内皮生长因子（VEGF）基因启动子的作用，探讨在低氧诱导视网膜新生血管形成中HPA对VEGF基因转...; 关键词：人视网膜血管内皮细胞（Human retinal MICROVASCULAR ENDOTHELIAL cells HRECs）乙酰肝素酶（Heparanase HPA）血管内皮生长因子（Vascular ENDOTHELIAL growth factor VEGF）染色质免疫沉淀法（Chromatin IMMUNOPRECIPITATION ChIP）

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