Morphological determination of localization and function of Golgi proteins  

作　　者：Yusheng Xing Yannan Jian Xiaodan Zhao Yue Zhang Zhenqian Zhang Xing Zhang Xiaoyan Zhang 

机构地区：[1]College of Biomedicine and Health,College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Biophysics Reports》2024年第2期121-132,共12页生物物理学报（英文版）

基　　金：supported by a grant from the National Natural Science Foundation of China to X.Zhang(32070693)。

摘　　要：In animal cells,the Golgi apparatus serves as the central hub of the endomembrane secretory pathway.It is responsible for the processing,modification,and sorting of proteins and lipids.The unique stacking and ribbon-like architecture of the Golgi apparatus forms the foundation for its precise functionality.Under cellular stress or pathological conditions,the structure of the Golgi and its important glycosylation modification function may change.It is crucial to employ suitable methodologies to study the structure and function of the Golgi apparatus,particularly when assessing the involvement of a target protein in Golgi regulation.This article provides a comprehensive overview of the diverse microscopy techniques used to determine the specific location of the target protein within the Golgi apparatus.Additionally,it outlines methods for assessing changes in the Golgi structure and its glycosylation modification function following the knockout of the target gene.

关 键 词：Golgi apparatus Golgi fragmentation IMMUNOFLUORESCENCE Super-resolution microscopy Electron microscopy Lectin staining GLYCOSYLATION 

分 类 号：Q244[生物学—细胞生物学]