基于紫胶红素的靶标有色化免疫层析试纸条联检大肠杆菌O157∶H7和沙门氏菌  

Immunochromatographic Test Strips for Simultaneous Detection of Escherichia Coli O157∶H7 and Salmonella Based on Shellac Red Conjugate

作　　者：刘坤[1] 王英林 黄志强[1] 刘箐[1] LIU Kun;WANG Yinglin;HUANG Zhiqiang;LIU Qing(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093

出　　处：《工业微生物》2024年第3期104-110,共7页Industrial Microbiology

基　　金：国家重点研发计划高信噪比功能集成探针创制技术研究(2023YFF1105002)。

摘　　要：为摆脱免疫层析试纸条对配对抗体的依赖,文章设计了一种新型靶标有色化免疫层析试纸条,使用紫胶红素和十二合水硫酸铝钾媒染剂,通过先媒后染的方法,实现了免疫层析试纸条的“可视化”。验证结果显示,该试纸条对大肠杆菌O157∶H7和沙门氏菌的检测限均达到了106 CFU/mL;同时,与常见食源性致病菌间不存在交叉反应,在鸡胸肉、鸡蛋、牛奶等食品基质中能够在增菌9 h内检出。文章成功设计了一种操作简单、检测成本低,更易国产化的快速检测免疫层析试纸条,为后续免疫层析试纸条的设计提供了新思路。In order to get rid of the need for paired antibodies in immunochromatographic test strips,this paper designed a new type of target-colored immunochromatographic test strips.The immunochromatographic strips were"visualized"by the method of mordanting and dyeing with shellac red pigment and potassium aluminum sulfate dodecahydrate.The results showed that the limits of detection for Escherichia coli O157∶H7 and Salmonella were 106 CFU/mL,there was no cross-reactivity with common foodborne pathogens,and the two were detectable within 9 hours of enrichment in chicken breast,egg,and milk food matrices.This paper successfully establish a simple,low-cost,and more easily localized rapid detection immunochromatographic test strip,and provided a new approach for the design of subsequent immunochromatographic test strips.

关 键 词：大肠杆菌O157∶H7 沙门氏菌 紫胶红素 靶标有色化 免疫层析试纸条 

分 类 号：TS207.4[轻工技术与工程—食品科学]