黑龙江省沙门氏菌血清型鉴定及两种血清分型方法对比  

Identification of Salmonella Serotypes in Heilongjiang Province and Comparison of Two Serotyping Methods

作　　者：李鑫[1] 杨红霞 仲伟麒 任启智 白晶[1] 张宇亮 季丽男 LI Xin;YANG Hongxia;ZHONG Weiqi;REN Qizhi;BAI Jing;ZHANG Yuiang;JI Linan(Heilongjiang Provincial Center for Disease Control and Prevention,Harbin 150030,China;Harbin Songbei District Center for Disease Prevention and Control,Harbin 150030,China;Infectious Disease of Heilongjiang Province,Harbin 150030,China)

机构地区：[1]黑龙江省疾病预防控制中心,黑龙江哈尔滨150030 [2]哈尔滨市松北区疾病预防控制中心,黑龙江哈尔滨150030 [3]黑龙江省传染病防治院,黑龙江哈尔滨150030

出　　处：《食品安全导刊》2024年第16期83-90,共8页China Food Safety Magazine

基　　金：2023年度黑龙江省卫生健康委科技计划“利用基因组学方法建立一套沙门氏菌血清分型体系的研究”(20231212070191)。

摘　　要：目的:分析沙门氏菌血清型分子鉴定试剂盒的分型效果,探寻一种新型高效的血清型鉴定方法。方法:采用沙门氏菌血清分子鉴定方法和经典血清凝集法分别对从黑龙江省食品监测和食源性疾病人群分离得到的69株沙门氏菌、37株沙门氏菌标准菌株以及往年分离并保藏的100株不同血清型沙门氏菌进行分型鉴定,对比两种分型方法的结果一致性。结果:两种分型方法成功率均为100%,结果的符合率为99.5%。其中来自黑龙江省的疾病人群分离的69株沙门氏菌共有10个血清型。结论:沙门氏菌血清型分子鉴定试剂盒与经典分型方法的一致性较高,且操作简便、准确性高,有望得到广泛应用。Objective:To analyze the typing effect of Salmonella serotype molecular identification kit and to explore a new and efficient serotype identification method.Method:69 strains of Salmonella,37 standard strains of Salmonella and 100 strains of Salmonella serotypes isolated and preserved in previous years were identified by using Salmonella serum molecular identification method and classical serum agglutination method,respectively,and the results of the two classification methods were compared.Result:The success rate of both classification methods was 100%,and the coincidence rate of the results was 99.5%.Among them,69 strains of Salmonella isolated from the disease population in Heilongjiang province had a total of 10 serotypes.Conclusion:The Salmonella serotype molecular identification kit has high consistency with the classical typing method,and is easy to operate and highly accurate,which is expected to be widely used.

关 键 词：沙门氏菌 血清分型 鉴定 

分 类 号：R446.5[医药卫生—诊断学]