益生菌微囊化抗逆性能研究  

Study on the Stress Resistance of Probiotic Microencapsulation

作　　者：王诚 孔德玲 董桂红 王玲 范秋苹 丁博群 WANG Cheng;KONG Deling;DONG Guihong;WANG Ling;FAN Qiuping;DING Boqun(Shandong Jianyuan Biotechnology Co.,Ltd.,Taian 271000,China)

机构地区：[1]山东健源生物科技有限公司,山东泰安271000

出　　处：《现代食品》2024年第9期82-86,共5页Modern Food

基　　金：山东省科技型中小企业创新能力提升工程(2022TSGC1272)。

摘　　要：以未微囊化的游离益生菌为对照,研究一种新型益生菌微囊化包被在不同胁迫条件下的生长存活情况。从长期储存、高温高湿、酸性环境等方面验证了微囊化包被技术有助于益生菌在恶劣环境下保持活力和增强稳定性。微囊化植物乳植杆菌和长双歧杆菌的常温长期储存存活率比未微囊化对照组分别提高79.02%和70.73%,差异显著(P<0.05)。高温高湿环境下,未微囊化包被的菌粉几乎完全失活。随着高温处理时间的增加,微囊化植物乳植杆菌和长双歧杆菌存活率分别为32.14%和40.36%。在固定湿度65%条件下,随着储存时间的增加,微囊化的植物乳植杆菌仍有14.29%的存活率,长双歧杆菌仍有10.54%的存活率。在模拟胃液中,处理120 min后,微囊化植物乳植杆菌和长双歧杆菌的存活率比菌粉组分别提高了59.22个百分点(P<0.05)和64.24个百分点(P<0.05)。在模拟肠液中,处理120 min后,微囊化植物乳植杆菌和长双歧杆菌的存活率比菌粉组分别提高了34.80个百分点(P<0.05)和33.82个百分点(P<0.05)。因此,微囊化包被技术能提高益生菌在恶劣环境下的存活力,增强抗逆性。To study the growth and survival of microencapsulated probiotics under different stress conditions,unmicroencapsulated probiotics were used as control.It was verified that the microencapsulation technology was helpful to maintain the vitality and enhance the stability of probiotics.The long-term storage survival rates of microencapsulated Lactiplantibacillus plantarum and Bifidobacterium longum were 79.02%and 70.73%higher than unmicroencapsulated,with significant differences(P<0.05).With the increase of high temperature treatment time,the survival rates of Lactiplantibacillus plantarum and Bifidobacterium longum microencapsulated were 32.14%and 40.36%.Under the condition of fixed humidity of 65%,with the increase of storage time,the survival rate of microencapsulated Lactiplantibacillus plantarum and Bifidobacterium longum was 14.29%and 10.54%respectively under the fixed humidity of 65%.After 120 minutes of treatment in simulated gastric juice,the survival rates of microencapsulated Lactobacillus plantarum and Bifidobacterium longum increased by 59.22 percentage points(P<0.05)and 64.24 percentage points(P<0.05)respectively compared to the bacterial powder group.After 120 minutes of treatment in simulated intestinal fluid,the survival rates of microencapsulated Lactobacillus plantarum and Bifidobacterium longum increased by 34.80 percentage points and 33.82 percentage points respectively compared to the bacterial powder group(P<0.05).Therefore,the microencapsulation technology can improve the viability of probiotics in harsh environment and enhance the stress resistance of probiotics.

关 键 词：植物乳植杆菌 长双歧杆菌 益生菌 微囊化 存活率 

分 类 号：TS201.3[轻工技术与工程—食品科学]