响应面法优化诺尔斯链霉菌发酵条件以提高谷氏菌素产量  

Optimization of fermentation conditions of Streptomyces noursei using response surface methodology to improve the gougerotin yield

作　　者：宋巍 许春丽[1] 施李鸣[1] 陈丹[3] 段小莉 李亚宁[2] 葛蓓孛[1] SONG Wei;XU Chunli;SHI Liming;CHEN Dan;DUAN Xiaoli;LI Yaning;GE Beibei(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;National Engineering Research Center for Agriculture in Northern Mountainous Areas,Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province,College of Plant Protection,Hebei Agricultural University,Baoding 071001,Hebei Province,China;Key Laboratory of Tobacco Pest Monitoring and Integrated Management in Tobacco Industry,Institute of Tobacco Research,Chinese Academy of Agricultural Sciences,Qingdao 266101,Shandong Province,China;Hebei Green Agricultural Science and Technology Group Co.Ltd.,Langfang 065000,Hebei Province,China)

机构地区：[1]中国农业科学院植物保护研究所植物病虫害综合治理全国重点实验室,北京100193 [2]河北农业大学植物保护学院,河北省农作物病虫害生物防治技术创新中心国家北方山区农业工程技术研究中心,河北保定071001 [3]中国农业科学院烟草研究所烟草行业烟草病虫害监测与综合治理重点实验室,山东青岛266101 [4]河北中保绿农作物科技有限公司,河北廊坊065000

出　　处：《农药学学报》2024年第3期517-525,共9页Chinese Journal of Pesticide Science

基　　金：烟草行业烟草病虫害监测与综合治理重点实验室开放课题(KLTPMIMT2022-13);中国烟草总公司重大科技项目(110202201019(LS-03))。

摘　　要：诺尔斯链霉菌Streptomyces noursei的次级代谢产物丰富,抑菌谱广,在农业上具有潜在的应用前景。作者实验室筛选到一株诺尔斯链霉菌S. noursei NK27,其次级代谢产物对番茄灰霉病的防治效果显著。为了提高NK27次级代谢产物中谷氏菌素的产量,以NK27发酵液中谷氏菌素产量为响应值,采用单因素试验、Plackett-Burman (PB)试验、最陡爬坡试验以及响应面法中的Box-Behnken试验设计,对NK27的发酵条件进行了优化。结果表明,诺尔斯链霉菌种龄、接种量及初始pH值3个因素对发酵效果影响最为显著。优化后的发酵条件为:初始pH值7.6,种龄32.6 h,接种量2.6%,转速220 r/min,发酵时间144 h。与原始发酵条件相比,优化后谷氏菌素含量提高了约3倍。本研究可为诺尔斯链霉菌大规模工业化发酵提供理论依据和技术支撑。Streptomyces noursei was rich in secondary metabolites and had a broad-spectrum antifungal activity. A strain of S. noursei NK27, whose secondary metabolites had significant control effect on Botrytis cinerea was screened in our laboratory. In order to improve the ability of NK27 to produce gougerotin, single-factor tests, Plackett-Burman (PB) tests, the steepest climb tests and Box-Behnken tests were designed to optimize the fermentation conditions of NK27. From the five fermentation conditions, the three factors of inoculum age, inoculation amount and the initial pH showed the most significant influence on the yield of gougerotin. The fermentation conditions after the optimization of response surface were as follows: initial pH 7.6, inoculum age 32.6 h, inoculation amount 2.6%, rotation speed 220 r/min, fermentation time 144 h. The content of gougerotin was 3 times higher than that under the original fermentation condition. This study provided a theoretical basis for the industrial fermentation of S. noursei.

关 键 词：响应面法 诺尔斯链霉菌 发酵条件 次级代谢产物 谷氏菌素 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ458