High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein  

作　　者：Haorong He Yuanhang Wei Yuqi Chen Xiyu Zhao Xiaoxu Shen Qing Zhu Huadong Yin 

机构地区：[1]Key Laboratory of Livestock and Poultry Multi‑Omics,Ministry of Agriculture and Rural Affairs,College of Animal Science and Technology,Sichuan Agricultural University,Chengdu,Sichuan 611130,China [2]Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu,Sichuan 611130,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第3期971-986,共16页畜牧与生物技术杂志（英文版）

基　　金：This research was funded by The National Key Research and Development Program of China,grant number 2021YFD1300600;China Agriculture Research System of MOF and MARA,grant number CARS-40;Sichuan Science and Technology Program,grant number 2021YFYZ0007,2021YFYZ0031 and 2022YFYZ0005;National Natural Science Foundation of China Grants,grant number 31972543.

摘　　要：Background The reproductive performance of chickens mainly depends on the development of follicles.Abnor-mal follicle development can lead to decreased reproductive performance and even ovarian disease among chick-ens.Chicken is the only non-human animal with a high incidence of spontaneous ovarian cancer.In recent years,the involvement of circRNAs in follicle development and atresia regulation has been confirmed.Results In the present study,we used healthy and atretic chicken follicles for circRNA RNC-seq.The results showed differential expression of circRALGPS2.It was then confirmed that circRALGPS2 can translate into a protein,named cir-cRALGPS2-212aa,which has IRES activity.Next,we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and HMGB1.Conclusions Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.

关 键 词：Apoptosis AUTOPHAGY CHICKEN CircRALGPS2 Follicle atresia PARP1 RNC-seq 

分 类 号：S831[农业科学—畜牧学]