Excess dietary Lys reduces feed intake, stimulates jejunal CCK secretion and alters essential and non‑essential blood AA profile in pigs  

作　　者：Maximiliano Muller Elout Van Liefferinge Alan Tilbrook Robert van Barneveld Eugeni Roura 

机构地区：[1]Centre for Nutrition and Food Sciences,Queensland Alliance for Agriculture and Food Innovation,The University of Queensland,Brisbane,Queensland 4072,Australia [2]Laboratory of Animal Nutrition and Animal Product Quality(LANUPRO),Department of Animal Sciences and Aquatic Ecology,Ghent University,339000 Ghent,Flanders,Belgium [3]Centre for Animal Science,Queensland Alliance for Agriculture and Food Innovation and the School of Veterinary Science,The University of Queensland,Brisbane,Queensland 4072,Australia [4]SunPork Group,Brisbane,Queensland 4009,Australia

出　　处：《Journal of Animal Science and Biotechnology》2024年第3期1336-1346,共11页畜牧与生物技术杂志（英文版）

基　　金：This study was partially supported by The University of Queensland and Australian Pork Limited as part of the project APL 2016/053.

摘　　要：Background Commercial diets are frequently formulated to meet or exceed nutrient levels including those of lim-iting essential amino acids(AA)covering potential individual variations within the herd.However,the provision of dietary excess of AA,such as Lys,may lead to reduced appetite and growth in pigs.The mechanisms modulat-ing these responses have not been extensively investigated.This study evaluated the effect of Lys dietary excesses on performance and satiety biomarkers in post weaning pigs.Methods Twenty-four pigs aged 21 d and weighing 6.81±0.12kg(mean±SEM)were individually housed and offered 1 of 4 dietary treatments for 3weeks:a diet containing a standardized ileal digestible Lys reaching 100%(T0),120%(T1),150%(T2)or 200%(T3)of the NRC(2012)requirements.At the end of the experiment,blood samples from the cephalic vein of the T0 and T3 groups were obtained for AA analysis.In addition,primary intestinal cultures from T0 pigs were used,following their humane killing,to evaluate the effect of Lys on gut hormone secretion and AA sensors gene expression under ex vivo conditions.Results Feed intake was linearly reduced(P<0.001)and the weight gain to feed ratio reduced(P<0.10)with increased dietary levels of Lys during the third-and first-week post weaning,respectively.Cholecystokinin con-centration(P<0.05)and the metabotropic glutamate receptor 1 and the solute carrier family 7 member 2(P<0.10)gene expression was enhanced in proximal jejunum tissues incubated with Lys at 20mmol/L when compared to the control(Lys 0mmol/L).Plasma Lys and Glu(P<0.05)concentration increased in the T3 compared to T0 pigs.In contrast,plasma levels of His,Val,Thr,Leu(P<0.05)and Gln(P<0.10)were lower in T3 than T0 pigs.Conclusion The present results confirm that excess dietary Lys inhibits hunger in pigs.Moreover,the results provide evidence of pre-and post-absorptive mechanisms modulating these responses.Lys dietary excesses should be nar-rowed,when possible,to avoid negative effects of the AA on appetite in pigs.

关 键 词：Amino acid BLOOD CHOLECYSTOKININ Feed intake LYSINE Pig SATIETY 

分 类 号：S828.5[农业科学—畜牧学]