月桂酸单甘油酯对大口黑鲈生长性能、肌肉营养品质及肠道健康的影响  

Effects of Glycerol Monolaurate on Growth Performance,Muscle Nutritional Quality and Intestinal Health of Micropterus salmoides

作　　者：张毅 刘滔 周秀珍 王扬 赵敏洁[4] 王旭堂 黄菊 冯凤琴[4] ZHANG Yi;LIU Tao;ZHOU Xiuzhen;WANG Yang;ZHAO Minjie;WANG Xutang;HUANG Ju;FENG Fengqin(School of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,China;Xianghu Laboratory,Hangzhou 311231,China;Zhejiang Provincial Aquatic Technology Promotion Station,Hangzhou 311110,China;College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China;Hangzhou Longyu Biotechnology Co.,Ltd.,Hangzhou 310000,China)

机构地区：[1]浙江海洋大学食品与药学学院,舟山316022 [2]湘湖实验室,杭州311231 [3]浙江省水产技术推广总站,杭州311110 [4]浙江大学生物系统工程与食品科学学院,杭州310058 [5]杭州龙宇生物科技有限公司,杭州310000

出　　处：《动物营养学报》2024年第6期3892-3902,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：浙江大学-恒泽领雁合作项目子课题(2022C02040)。

摘　　要：本试验旨在研究月桂酸单甘油酯(GML)对大口黑鲈生长性能、肌肉营养品质及肠道健康的影响。试验选取体重为(37.81±1.12) g的大口黑鲈幼鱼1 200尾,随机分为4组,每组3个重复,每个重复100尾。对照组投喂基础饲料,试验组分别投喂在基础饲料中添加0.50、0.75和1.00 g/kg GML的试验饲料。试验期130 d。结果表明:1)与对照组相比,0.50和0.75 g/kg GML组的体长和形体比显著提高(P<0.05),饲料系数和肥满度显著降低(P<0.05)。2)与对照组相比,0.75 g/kg GML组的肠道胰蛋白酶活性显著提高(P<0.05),0.50、0.75和1.00 g/kg GML组的肠道脂肪酶活性显著提高(P<0.05)。3)与对照组相比,0.50和0.75 g/kg GML组的血清碱性磷酸酶和超氧化物歧化酶活性显著提高(P<0.05);0.50、0.75和1.00 g/kg GML组的血清溶菌酶含量显著提高(P<0.05),血清丙二醛含量显著降低(P<0.05)。4)与对照组相比,0.50、0.75和1.00 g/kg GML组的肌肉饱和脂肪酸含量显著降低(P<0.05),肌肉多不饱和脂肪酸含量显著提高(P<0.05),肌肉谷氨酸、赖氨酸、组氨酸和总风味氨基酸含量显著提高(P <0.05)。由此可见,饲料中添加0.50和0.75 g/kg GML对大口黑鲈的生长性能、肌肉营养品质及肠道健康均有明显的促进作用,表明GML作为功能性饲料添加剂具有良好的应用前景。The purpose of this experiment was to investigate the effects of glycerol monolaurate(GML)on growth performance,muscle nutritional quality and intestinal health of Micropterus salmoides.A total of 1200 juvenile Micropterus salmoides with body weight of(37.81±1.12)g were randomly divided into 4 groups with 3 replicates per group and 100 fish per replicate.The control group was fed a basal diet,and the experimental groups were fed the basal diets supplemented with 0.50,0.75 and 1.00 g/kg GML,respectively.The experiment lasted for 130 days.The results showed as follows:1)compared with the control group,the body length and shape ratio of 0.50 and 0.75 g/kg GML groups were significantly increased(P<0.05),and the feed coefficient and condition factor were significantly decreased(P<0.05).2)Compared with the control group,the intestinal trypsin activity of 0.75 g/kg GML group was significantly increased(P<0.05),and the intestinal lipase activity of 0.50,0.75 and 1.00 g/kg GML groups were significantly increased(P<0.05).3)Compared with the control group,the serum alkaline phosphatase and superoxide dismutase activities of 0.50 and 0.75 g/kg GML groups were significantly increased(P<0.05);the serum lysozyme content of 0.50,0.75 and 1.00 g/kg GML groups was significantly increased(P<0.05),and the serum malondialdehyde content was significantly decreased(P<0.05).3)Compared with the control group,the muscle saturated fatty acid content of 0.50,0.75 and 1.00 g/kg GML groups was significantly decreased(P<0.05),muscle polyunsaturated fatty acid content was significantly increased(P<0.05),and the muscle glutamate,lysine,histidine and total flavor amino acid contents were significantly increased(P<0.05).In conclusion,the addition of 0.50 and 0.75 g/kg GML in diet can promote the growth performance,muscle nutritional quality and intestinal health of Micropterus salmoides,which indicate that GML has a good application prospect as a functional feed additive.

关 键 词：月桂酸单甘油酯 大口黑鲈 生长性能 营养品质 肠道健康 

分 类 号：S963.73[农业科学—水产养殖]