PD-L1不同克隆抗体在胃腺癌组织中表达的一致性分析  

Consistent analysis of the expression of different PD-L1 antibodies in gastric adenocarcinoma

作　　者：周文斌 陈雪 付尧[1] 吴鸿雁[1] 王朝珊 孙琦[1] ZHOU Wenbin;CHEN Xue;FU Yao;WU Hongyan;WANG Chaoshan;SUN Qi(Department of Pathology,Nanjing Drum Tower Hospital,the Affiliated Hospital of Nanjing University Medical School,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属鼓楼医院病理科,南京210008

出　　处：《临床与实验病理学杂志》2024年第6期597-602,共6页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨胃腺癌组织中程序性细胞死亡配体1(programmed cell death ligand-1,PD-L1)4种克隆号抗体免疫组化(IHC)检测的一致性。方法对286例胃腺癌手术样本组织芯片(tissue microarray,TMA)进行PD-L122C3、SP263、E1L3N和SP1424种克隆号抗体染色,采用肿瘤细胞比例评分(TPS)和联合阳性评分(CPS)标准,在不同临界值下对抗体的一致性进行统计学分析。结果22C3在4种克隆号抗体TPS(临界值1%、5%、10%)和CPS(临界值1、5、10)评估下的阳性率均最高。一致性分析显示,仅在CPS阳性临界值为1时,E1L3N与SP142(κ=0.612)和SP263(κ=0.660)的一致性较好,其余情况下各抗体之间具有中等或以下的一致性。结论PD-L14种克隆号抗体在不同TPS和CPS阳性临界值下的一致性不佳,在胃腺癌临床检测时不建议互换试剂,应按照规范描述检测结果。Purpose The purpose of this study was to investigate programmed death ligand-1(PD-L1)expression in gastric adenocarcinoma(GAC)patients and the consistency of immunohistochemical(IHC)detection of four different clones of PD-L1 antibodies,so as to provide reference for the gradual standardization of PD-L1 IHC detection in gastric adenocarcinoma and subsequent clinical studies.Methods This study collected surgically resected and pathologically confirmed specimens from 286 gastric adenocarcinoma patients,and specimens were stained with four antibodies:PD-L122C3,SP263,E1L3N and SP142.The consistency of the antibodies was statistically analyzed under different critical values using two scoring criteria:comprehensive positive score(CPS)and tumor proportion score(TPS).Results Regardless of the TPS and CPS cut-off values,the positive rate of 22C3 was the highest among the four antibodies.Consistency analysis showed that E1L3N had good consistency with SP142(κ=0.612)and SP263(κ=0.660)only when the cut-off value of CPS positive was 1.In addition,the consistency between the other antibodies was only moderate or poor.Conclusion The four PD-L1 antibodies exhibited inconsistent concordance across various TPS and CPS positive thresholds,so it is not recommended to exchange reagents during clinical testing of GAC and the test results shall be documented in accordance with the specification.

关 键 词：胃腺癌 PD-L1 一致性分析 

分 类 号：R735.2[医药卫生—肿瘤]