胶原蛋白肽功能饮料的贮藏稳定性及体内抗血栓活性评价  

Storage Stability and in Vivo Antithrombotic Activity Evaluation of Collagen Peptide Functional Beverage

作　　者：梁芮涵 李博[1] LIANG Ruihan;LI Bo(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《现代食品科技》2024年第6期178-188,共11页Modern Food Science and Technology

基　　金：国家大宗淡水鱼产业技术体系(CARS-45)。

摘　　要：抗血小板活性功能食品可以代替药物在血栓的预防中发挥作用。前期研究表明,鲢鱼皮胶原蛋白肽(Silver Carp Skin Collagen Peptides,SCPs)具有良好的抗血小板活性,开发基于SCPs的饮料有望起到预防血栓的作用。该研究探究SCPs粉末和饮料在不同贮藏条件下的稳定性,并采用尾血栓模型对贮藏前后的SCPs粉末和饮料进行体内抗血栓活性评价。结果显示,真空包装的SCPs粉末常温(25℃)贮藏两个月肽损失率约为1.43%;SCPs饮料常温贮藏三个月肽损失率约为5.90%,高温(37℃)贮藏三个月肽损失率约为12.87%,且伴随着美拉德反应、菌落数的生长和感官品质的下降;SCPs粉末在200 mg/kg.bw的饲喂剂量下对小鼠尾血栓形成的抑制率为66.4%,常温贮藏两个月后的SCPs粉末相较于贮藏前体内活性未发生显著下降;SCPs饮料对小鼠尾血栓形成的抑制率为58.7%,高温贮藏三个月后抑制率降至45.3%,但仍能与25 mg/kg.bw饲喂剂量的阿司匹林作用效果相似。该研究表明,SCPs作为粉末或开发成液体饮料的产品形式均为可行方案,二者均可作为预防血栓的膳食补充剂。Antiplatelet functional foods can replace drugs in the prevention of thrombosis.Previous studies have shown that silver carp skin collagen peptides(SCPs)have a good antiplatelet activity,and the developed SCPs-based beverages are anticipated to play a role in the prevention of thrombus.In this study,the stability of SCPs powder and derived beverage under different storage conditions was investigated,and a tail thrombosis model was used to evaluate the antithrombotic activity of SCPs powder and derived beverage before and after the storage.The results showed that the peptide loss rate of the vacuum packed SCPs powder stored at room temperature(25℃)for two months was about 1.43%;The peptide loss rate of the derived SCPs beverage was about 5.90%after being stored at room temperature for three months,and was about 12.87%after being stored at a relatively high temperature(37℃)for three months,accompanied by the occurrence of the Maillard reaction,growth of bacterial colony and decline of sensory quality;The inhibitory rate of SCPs powder against the tail thrombus formation in mice was 66.4%when the feeding dose was 200 mg/kg·bw.The activity of SCPs powder after two months of storage at room temperature did not decrease significantly compared with that before the storage.The inhibitory rate of the derived SCPs beverage against the tail thrombosis in mice was 58.7%,which decreased to 45.3%after three months of the high-temperature storage,though a similar effect as that of aspirin at a dose of 25 mg/kg·bw was still detected.This study indicates that SCPs powder or liquid beverage are feasible product options,both of which can be used as dietary supplements for the prevention of thrombosis.

关 键 词：鲢鱼皮胶原蛋白肽 抗血小板 功能饮料 稳定性 小鼠尾血栓模型 

分 类 号：TS275.4[轻工技术与工程—农产品加工及贮藏工程]